| Molecularly imprinted polymers (MIPs) are a new kind of macromolecularmaterials with molecular recognition capability. The process of preparation consistsof:(1) mixing the template molecules with monomers with appropriate functionalgroups;(2) low temperature and photo polymerization, and extracting the templatemolecules from the polymer, which leaves cavities inside the polymers that arecomplementary in terms of size, shape, and functional group to those of the templatemolecules. Because MIPs have the advantages of predetermination, specificity andpracticability, they have been extensively used in chromatographic separation,antibody mimic, solid-phase extraction, biosensors, catalyzing reaction and otherelevant fields.The molecularly imprinted polymer prepared by traditional method is difficult tobe popularized because of its poor recognition performance, small adsorbed amountand the template molecular is hard to be eluted. In order to solve this problem, theMagnetic molecularly imprinted polymer microspheres, which naringenin and ostholewere chosen as template molecule, the magnetic ferroferric oxide as carrier, wereprepared by suspension polymerization method and characterized by UV and SEM.The prepared MIPMs were used as the stationary phase of Solid Phase Extraction(SPE) cartridges and the conditions were optimized. And the imprinting, mass transfer,recognition and adsorption mechanism of Osthole and naringenin MIPMs werefurther investigated. The contents of this thesis include the following aspects:1. Using the the magnetic ferroferric oxide as carrier, naringenin and osthole werechosen as template molecule, the AM and MAA as the functional monomer separately,DMF as the solvent, the magnetic molecularly imprinted polymer microspheres wereprepared by suspension polymerization method. The MIPMs with super paramagneticmaterials get rid of the operational complexity of traditional centrifugal technology.And the MIPMs prepared characterized by SEM.2. Studying on the combination of template polymer dynamicts, and the dynamicadsorption of naringenin molecular imprinting polymer. the selective bindingcharacteristics of the template polymer were evaluated by Scatchard analysis. Theresults show that: it is effectively solve the less adsorption capacity of molecularly imprinting technology, and the complex operation of solid liquid separation bysuspension polymerization method of core-shell super paramagnetic molecularyimprinted polyer. Dynamic adsorption of naringenin molecular imprinting polymerindicated the adsorption increased quickly before30min and reached saturation in ashort period of time about50min. The scatchard analysis shows that the dissociationconstant Kdand maximum apparent adsorption quantity Qmaxof these binding sites are8.47mmol/L and634.40μmol/g, respectively. The prepared naringenin-MIPMs wereused as the stationary phase of Solid Phase Extraction (SPE) cartridges and theconditions were optimized. The results show that naringenin-MIPMs exhibited higherselectivity and recognition ability for naringenin than non-imprinted polymer (NIPMs).the application of naringenin-MIPMs with high affinity and excellent stereo-selectivitytoward naringenin in SPE might offer a new method for the enrichment anddetermination of naringenin compounds in the natural products.3. Studying osthole naringenin MIPMs’s adsorption and the recognitionperformance by transmission electron microscopy (TEM).The results show thatosthole naringenin MIPMs. Dynamic adsorption of naringenin molecular imprintingpolymer indicated the adsorption increased quickly before45min and reachedsaturation in a short period of time about60min. The scatchard analysis shows thatthe dissociation constant Kdand maximum apparent adsorption quantity Qmax ofthese binding sites are7.2mmol/L and420.97μmol/g, respectively. At the same time,saturation magnetization of osthole naringenin magnetic molecularly imprintedpolymer was10.378emu/g, it can be separation quickly in additional magnetic field. |
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