Isolation And Characterization Of A4-Fluorocinnamic Acid-Degrading Bacteria

A highly effective4-fluorocinnamic acid (4-FCA) degrading bacterial strain HZF1, was isolated from an agrochemicals factory. Based on its16S rDNA gene sequence analysis, physiological and biochemical characteristics, the strain was identified as a Rhodococcus sp.Strain HZF1could use glucose, starch,β-cyclodextrin, polysorbate40,4-FCA,4-fluorobenzoic acid (4-FBA),4-fluorophenol as sole carbon sources for growth. It tested negative for gram, oxidase, starch hydrolysis and methyl red and tested positive for vogues prausker. Cells are resistant to spectinomycin (50mg L-1) and kanamycin (50mg L-1), but susceptible to chloramphenicol (50mg L-1), ampicillin (50mg L-1) and streptomycin (50mg L-1)The optimal temperature and pH for strain HZF1growth and biodegradation were30℃and7.0, respectively. Strain HZF1could degrade approximately100%of200,500mg/L of4-FCA within12,24h, respectively. When the4-FCA concentration was1000mg/L, Strain HZF1began to degrade after12hours’adaption time, and the degrdration was finished in36h. while the concentration was up to2000mg/L, the adaptation time extended to24h, and3d was needed to degrade completely. With the add of glucose, the degradation of4-FCA was slow dowm and strain HZF1ability of degrade was increased later.The intermediate metabolites were detected by LC-MS, NMR and IC. The pathway of4-FCA degradation by strain HZF1was proposed as follow:the4-FCA converted into4-FBA through the side chain scission; the4-FBA was degraded into carbon dioxide and H2O by ring-opening and fluoride ion was removed at the same time.There are less report about4-FCA-degrading by microorganism in the intermational and this is the first report of4-FCA-degrading strain from the genus of Rhodococcus. It is a great significance for the follow-up research in the biodegradation of4-FCA.