Synthesis And Properties Study Ofrhodamine Derivatives

In recent years, the design and development of fluorescent probes for the detection of transition-metal ions have received considerable attention because fluorescent chemosensors have several advantages over otherm ethods such as high sensitivity, selectivity, and real-time monitoring. Fe3+play a important role in the human body, content is too high and too low will cause some disease, so developing a right method for detecting Fe3+is particularly important. FRET is an active field in supramolecular chemistry because its potential practical benefits in cell physiology, as well as selective and sensitive sensing toward targeted molecular or ionic species.Herein we describe our results concerning a sensor based on Rhodamine B, in order to FRET nature as the main line, three kinds of compounds in the testing of the system, and the results show that the three probes has excellent optical properties, and all the structure of the compound by NMR, TOF-MS, and IR was carried out.These results indicate the high selectivity of probe RPC for Fe3+, Fe3+binding with probe RPC caused the ring-opening of rhodamine and produce a color change from colorless to pink, this implied that RPC allowed naked-eyed detection for Fe3+. A clear fluorescent emission was observed around580nm in the presence of Fe3+. The formation of a1:1RPC-Fe3+complex was confirmed by the titration curve and JOB curve. The probe RPC can be used as a fluorescent probe for detecting Fe3+.The probe RD also has a good response to the Fe3+, but compared with RPC, RD is a green fluorescent, through further tests found that dansyl chloride and ring-opening of rhodamine that can happened FRET, wich excitation dansyl chloride can see rhodamine emission peak, thus avoiding the small stokes shift of rhodamine. RD allowed naked-eyed detection for Fe3+, as the nature of the fluorescent probe RD is more better than RPC.The probe RDT dual sensor selectively detects Hg2+and Fe3+, compare with RPC and RD, the RDT have three kinds of fluorophore, indole from tryptophan to dansyl chloride, from dansyl chloride to rhodamine happened double FRET. When the RDT combined with Hg2+, it shows completely quenched, this because the Hg2+quenching the dansyl chloride groups so that energy cannot be passed. The RDT is green fluorescence, RDT can open hole testing Fe3+. The formation of a1:1RPC-Fe3+and RPC-Hg2+complex were confirmed by the titration curve and JOB curve.