Study On Development Of Ganoderma Lucidum And Polygonum Multiflorum Thunb Wine Spirits And Its Antioxidant Activity In Vitro

This thesis was aim at using Ganoderma Lucidum and Polygonum multiflorum Thunb asmain materials, complemented with Radix Angelicae Sinensis, Rehmannia Glutinosa Libosch.etc. to produce liquor with good appearance, taste and aroma, meanwhile putting forward thefeasible manufacture technology. The soaking process of Chinese herbal medicine, the extractof effective components, the scheme of blending were optimized. Finally, a kind of liquorwith stable quality and acceptable flavor was attained. The antioxidant activities of the liquorwere evaluated.The effect of alleviating dysmenorrheal were discussed. The following resultswere obtained.Water extraction and alcohol extraction were applied to the Ganoderma Lucidumpolysaccharides from Ganoderma Lucidum. The results showed that the higher content ofGanoderma Lucidum polysaccharides in water extraction. In order to get higher qualityGanoderma Lucidum immersion of spirit, Ganoderma Lucidum was immersed in alcohol toget alcohol leaching extract first, and then the residue was immersed in water, concentrated toextractum, dissolving water extract soluble in low-alcohol wine. Through this way,Ganoderma Lucidum could be used fully.The optimal conditions to extract the effective ingredient stilbene glycoside inPolygonum multiflorum Thunb were as follows: temputerature of30℃,50%(V/V) of alcohol,20:1solid/liquid ratio and soaking twice for5days. Under this condition, the soluble solidscontent of Polygonum multiflorum Thunb leaching also were reached at a better level.The different kinds of Chinese herbal medicines were extracted based on the condition ofPolygonum multiflorum Thunb, obtaining the soak of the medicinal material leaching time for4~5days, in this case, the soluble solids content could reach at better level.Different leach liquors were optimized by an L9(34) orthogonal test. Results showed thatthe extract of Ganoderma Lucidum:(Polygonum multiflorum Thunb and Lycium barbarum):other Chinese herbal medicines=3:5:5(v/v/v), and available color, transparency and flavormixtures were attained. The clarified mixtures were optimized by orthogonal test. The results showed that theoptimal blending Ganoderma Lucidum and Polygonum multiflorum Thunb wine spirits wereas following:45%(V/V) of alcohol, citric acid0.4%,cane sugar8%.Then, the variousphysical and chemical index of the wine were detected.The antioxidant properties of wine were evaluated by using different antioxidation tests,including reducing power, DPPH radical scavenging, ABTS free radical, superoxide anionradical scavenging, hydroxyl radical scavenging. The results showed that the wine exhibitedstrong reducing capacity, DPPH radical scavenging, ABTS free radical, superoxide anionradical scavenging, hydroxyl radical scavenging. The wine has good antioxidant ability.Meanwhile, the wine has good effect on dysmenorrhea in remission.