| Cod skin collagen peptide is the enzymolysis product of cod skin collagen,which is hydrolyzed by kinds of protease. It has a variety of physiologic function,such as beauty and skin care, calcium supplements and strong tendon bones,hemostatic and immune function and so on. The collagen peptide is also supposed tohave the protective and healing effect on gastric mucosa, but there is no systematicresearch to provide a theoretical basis. In this paper, the collagen was extracted fromCod skin and hydrolyzed into peptide of different molecular weight. Then theprotective effect of different molecular weight Cod skin collagen peptide (CCP) ongastric mucosa is studied. And the possible mechanism is also revealed in this paperto provide a theoretical basis for the application of the collagen peptide.1. The collagen is extracted from Cod skin, combining the acid method and thehot water method. Using the compound enzyme to hydrolyze collagen and determingthe best enzymatic time to prepare the maximum amount of high molecular weight(Mw>8k Da), middle molecular weight (3k Da~8k Da) and low molecular weight(Mw<3k Da) CCP, that is respectively about15min,40min and2.5h. The ultrafiltration membrane of3k and8k MWCO is chosen to separate the hydrolyzate ofCod skin collagen and obtain different molecular weight CCP. After lyophilizationand composition analysis of CCP and collagen, we find that the purity of the sampleis more than80%, the average molecular weight of each sample is within themolecular weight segment they belong. Therefore, all the samples we prepared canmeet the requirements of animal experiment to the test substance.2. The animal model of ethanol induced acute gastric mucosal injury is used tostudy the preventative and protective effect of CCP on the acute injury of the gastricmucosa. The pathological results of the gastric tissue show that pre-given certaindose of CCP can effectively reduce the high concentration of ethanol-induced gastricmucosal injury index and protect the gastric mucosa. The effect of middle molecular weight CCP is best and similar to that of non-enzymatic collagen.3. CCP and collagen can play the preventative and the protective effect throughimproving the level of gastric mucosa barrier, to protect the gastric mucosa to reducethe hydrogen ions and the infiltration of inflammatory cells invasion. They also canenhance the GSH content and remove the free radical of the body effectively tomaintain high activity of antioxidase (SOD, GSH-PX) of gastric tissue, which canreduce attacks on the gastric mucosa injury by the oxidation reaction and itsproduction such as MDA caused by free radical.4. Using the acetic acid induced chronic gastric as the animal model to study theauxiliary repair effect on the gastric ulcer. The pathological results of the gastrictissue show that given certain dose of CCP and collagen after making the acetic acidmodel, the ulcer index is significantly reduced and the repair effect of gastric ulcer isapparently better than the model group which is not given the test sample. And theeffect of low molecular weight CCP is best and close to that of positive drug.5. Given CCP and collagen after acetic acid burning can raise the level of thegastric mucosal barrier to protect the ulcer to mitigate the further destruction of thegastric hydrogen ion. CCP can improve GSH content and effectively remove the freeradical in serum caused by acetic acid. The remove of free radical reduces the MDAand inducible NO content and maintains the high activity of SOD and GSH-PX inserum so as to relieve damage of ulcer tissue. The detection of mRNA expressionlevels of some repair factors related to ulcer repair in gastric tissue reveals that thegastric ulcer repair process involves kinds of growth factors and cytokines, includingvascular endothelial growth factor (VEGF), epidermal growth factor (EGF),transforming growth factor (TGF βl), heat shock protein70(HSP70) and immunecytokines (IL-1β and of IL-2). Among those factors, CCP can promote HSP70mRNA expression level to promote ulcer repair. Therefore, CCP can be able toachieve the auxiliary repair effect on the gastric ulcer by both reducing furtherdamage of ulcer and promote the expression of the repair factor in vivo. |
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