Studying Of Membrane Protein And Surface Enhanced Substrate By Surface-enhanced Infrared Absorption Spectroscopy

Surface Enhanced Infrared Absorption Spectroscopy (SEIRAS) can significantlyenhance the vibrational signals of adsorbed molecules on the metal surface due to theelectromagnetic couple. Since its high sensitivity and easy realization, it has become animportant surface analysis technique. This thesis mainly includes:1. Halobacterium salinarum sensory rhodopsin II (H.s.SRII) is a seven transmembrane-helical protein photoreceptor, in which the retinal chromophore binds to G-helical lysineresidue through a Schiff base(C=N), and it is dislikable to blue-green light. Under lightirradiation, microbial rhodopsin will be excited to several high-energy intermediates andthen come back the original through relaxing thermally. Here, we investigated thatlight-induced (470nm) sensory rhodopsin II had different intermediates and structurechanges after adding quantum dots (MSA-CdSe/ZnS QDs), checking by SEIDAS. We caninfer that the research might shed a light on the further in-depth studying biomembrane, inwhich the biomembrane protein is a very important part for assisting to transport substancesand communicate signals.2. SEIRS substrate prepared by chemical deposition of gold is limited to itsreproducibility and expensive materials. Here, we prepared an economical substrate byusing a kind of carbon nano-materials, which are always used in the electrochemistry, andmaking them connect with suitable gold nano-particles by electrostatic interaction. Thisexperimental method could get a concentration of nM-checking, and will be good forstudying other materials.