The Chemical Consitents Of Ligularia Kangtingensis

The genus Ligularia (Compositae) is a perennial herbaceous, mainly distributedin southwest and northwest China. Some species of this genus were used as ‘ziwan’, aTrational Chinese Medicine, with the effects of activating blood circulation to dissipateblood stasis, heat-clearing and detoxifying, eliminating phlegm and stopping cough,vomiting, diuretic and so on, which were commonly called ‘shanziwan’.Eremophilanolide-type sesquiterpenes and pyrrolizidine alkaloids are the maincomponents in this genus. Due to their multi-physiological functions, the research onchemical consituents of this genus was started since the60’s of last century, andachieved some important results. In this thesis, we studied the chemical constituents ofLigularia Kangtingensis S. W. Liu, a plant from the genus Ligularia, which wascollected from the Kangding county, Sichuan Provience. It is a meaningful work for usto find more worthwhile medicinal of genus Ligularia, develop the new natureresources and find new anti-tumor activity compounds with the application prospects.In this study, we extracted volatile oil of L. Kangtingensis by steam distillationmethod, and analysed its chemical composition by gas chromatography-massspectrometry (GC-MS).45compounds were isolated from L. Kangtingensis and30components were identified, which were accounting for79.61%of the total volatile oil.Among them, several of the major components were: β-elemene (4.67%),4-methylene-1-(1-methyl ethyl)-bicyclo [3.1.0]-hexane-3-acetate (5.45%), GemmaeneB (5.58%), eremophilene (11%) and β-eudesmene (15.53%).Volatile oils’s antibacterial activity, aganist Escherichia coli and Staphylococcusaureus, were evaluated by MTT colorimetric assay. The results showed that theinhibitory rates against E. coli and S. aureus were43.77%and55.09%respectivelywhen the concentration for volatile oil was20mg/ml. This experiment preliminarilyindicated that, the volatile oil of L. Kangtingensis has certain inhibition activitiesagainst gram-positive and gram-negative bacteria.For the non-volatile components of L. Kangtingensis, column chromatography(CC), preparative thin layer chromatography (PTLC), recrystallization were used for isolation, and NMR、MS、IR、ECD spectra were used for identification of the structures.18compounds were isolated, which were stearic acid (1), sitosterol (2), stearyl alcohol(3), Ligudetatin A (4), Liguhodgsonal (5), Ligukangtingonal (6), oplopanone (7),1,6-dihydroxy-4(15)-epoxyeudesmane (8), oleanolic acid (9), ursolic acid (10),octacosanol (11), taraxerol+lupeol (12), pomolic acid (13), taraxerol (14), oleic acid(15),8β-Ethoxyeremophil-3,7(11)-diene-8α,12(6α,15)-diolide (16), glycerol trioleate(17), vanillin (18). They belong to the sesquiterpene, triperenoids, fatty acidscompounds, and compound6is a new compound.