Preparation Of Collagen Peptide Powder And Calcium Malate Horse Bones

This research on smoked horse meat, smoked horse intestinal residual by-product of bone processingtechnology improvement, form with high added value products to improve the state of remote areasgenerally lack calcium status. Through process improvement, not only can reduce the waste of boneresources, but also gain greater economic benefits. This experiment uses raw horse bones for degreasingprocess, enzymatic hydrolysis preparation of collagen protein peptide powder, calcium malic acidpreparation process and the horse slag on osteoporosis prevention trial.(1)By single factor experiment design response surface optimization experiment to determine theoptimal skim conditions: solid-liquid ratio to1:4g/mL, degreasing temperature40℃, degreasing time9h.Thetotal protein content is26.38%to horse bones.(2)In response to the value of a degree of hydrolysis and nitrogen yield，the degree of hydrolysis of thealkaline protease is14.41%,nitrogen recovery is68.25%.It is select the alkaline protease.(3)It is start enzymatic reaction test. By single factor experiment and design response surfaceoptimization experiment to determine the optimal enzyme solution conditions: enzymolysis temperature of47℃, solid-liquid ratio to1:6g/mL, the pH value was9, enzyme amount to6200U/g and in the bestcondition Degree of hydrolysis is17.47%.(4)Calcium dissolution rate as index, by single factor test and orthogonal test to determine the bestmalic acid calcium preparation conditions: acid concentration of12g/100mL, extraction temperature is90℃, solid-liquid ratio is1:6g/mL, time2h, calcium dissolution rate is92.5%.(5)The horse collagen protein peptide powder meets the national standards and general health(functional) food inspection project standards. Horse ossein kda protein peptides are broken down into10KDA kda dispersed peptides.(6) Through rat osteoporosis trials show that calcium malate for osteoporosis prevention role.