Synthesis Of Water Dispersible Iron Oxide Nanoparticles Used In Doxorubicin Drug Loading

Iron oxide nanoparticles (NPs) were synthesized by thermal decomposition of the iron (III) acetylacetonate (Fe(acac)3) in different organic solvents with high boiling point. The samples were characterized with Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetric Analysis (TGA), X-ray Diffraction (XRD), particles&zeta potential Analyser, X-ray photoelectron spectroscopy analysis (XPS), Superconductivity Quantum Interference Device (SQUID) and Transmission Electron Microscopy (TEM). The alcohols with high boiling point, as well as polymers, were used as the reaction solvents. The NPs synthesized in different solvents had different properties. The addition of modifiers and altering of temperature also had great influence on the preparation of NPs. Additionally, we have investigated the drug loading and in virtro releasing of the NPs. The key points are as follows:(1) The size of NPs synthesized in triethylene glycol (TEG) was7.5±1.4nm, but the NPs could not perform colloidal stability in water for a long time. Addition of proper sodium dodecyl sulfate (SDS) could reduce the size of prepared NPs which performed poor water-solubility, and the water-solubility of NPs could be improved via modification of polyethyleneimine (PEI).(2) The size of NPs prepared in methoxy polyethyleneglycol (mPEG) with addition of polyvinylpyrrolidone (PVP) could be controled by adjusting temperature, ranging from10～24nm. The size of NPs was larger, however, which had fine crystal morphology and narrow size distribution.(3) The NPs coated with polyethyleneglycol (PEG) and PVP performed very good colloidal stability in deionizied water and several kinds of physiological buffers, and the size of NPs could be controled via altering the temperature of reaction and molecular weight of PVP, ranging from4to10nm.4.1nm sized NPs synthesized at200℃for8h had potential application in Ti contrast agent.8.8nm sized NPs prepared at260℃for1h had high cristallinity, which also had high encapsulation efficiency for doxorubicine (DOX), and the in vitro releasing rate of NPs@DOX showed pH sensitive, which was faster at lower pH.(4) By using1,4-butanediol or methyldigol as reaction solvent, there was non-magnetic jasper intermediate producting in preparation process of NPs, which could easily be changed to black magnetic materials by oxidization of air. In preliminary analysis, non-magnetic jasper intermediate was a particular compound containing with Fe2-, and black magnetic materials were magnetite.