Speciation Analysis Of Arsenic In Foods Of Animal Origin By High Performance Liquid Chromatography Coupled With Atomic Fluorescence Spectrometry

Element toxicity and biological importance are closely related to its chemicalspeciation. For arsenic compounds, inorganic arsenic is the most toxic, and organicarsenic, less toxicity, arsenobetaine and arsenocholine are often considered to benon-toxic. Therefore, in the determination of arsenic, speciation analysis is necessary.In recent years, the arsenic speciation analysis has become a hot issue for the field oflife sciences, environmental sciences, and clinical medicine and so on.The complexity of the sample matrix and the low concentration of the analytedecide that in most cases, arsenic speciation analysis needs highly selective separationtechniques and associated with the high sensitivity detection techniques. The researchdirection of the essay is the high performance liquid chromatography combined withhydride generation and atomic fluorescence spectrometry. Under the laboratoryconditions of animals, plants and food inspection center which belongs to TianjinEntry-Exit Inspection and Quarantine Bureau, the essay carrys out a study of thefollowing three aspects:1: presents the differential liquid chromatography-atomic fluorescence. To solvethe chromatographic peaks overlapped problem for the analysis of arsenite, arsenate,monomethyl arsenate and dimethyl arsenate, the paper presents the differential liquidchromatography-atomic fluorescence based on the vector algorithm. It improves thearsenic speciation standard curve correlation coefficients, conducive to an accurateanalysis of the actual sample.2: research the sample pretreatment method. With the increasing demand forarsenic speciation analysis, sample pretreatment technology gets more and moreattention. This paper compares the ultrasound centrifugation extraction andaccelerated solvent extraction, finally choose the accelerated solvent extraction as thesample preparation method, and optimize the accelerated solvent extraction conditions,finally the extraction efficiencies of arsenic species are greater than90%.3: Use high performance liquid chromatography combined with hydridegeneration and atomic fluorescence spectrometry to simultaneous determinatearsanilic，nitarsone and roxarsone residues in foods of animal origin. In recent years,with the extensive use of organic arsenic preparation, its residual safety problem hascaused widespread attention. This paper optimizes the high performance liquid chromatography separation conditions, hydride generation conditions and atomicfluorescence detection conditions. Under the instrument optimum conditions,simultaneous determinate arsanilic，nitarsone and roxarsone residues in four kinds ofcommercially available samples of chicken liver and pork liver. The experimentalresults show that the detection method has a good recovery and high stability, and therelative standard deviations (RSD) of the multiple experimental results are less than5%.