| The normal analytical techniques used in determination of phenols is mainly high-performance liquid chromatography (HPLC), especially the reversed-phase liquid chromatography (RPLC). However, in the analysis of practical samples, in order to obtain certain sensitivity, the substances interfered to the chromatographic analysis should be removed before the determination of phenols. Thus, sample preparation is a key step in quantitative analysis.Solid phase extraction technology has become an important sample pre-treatment technology at present. Moreover, sorbent for core of the solid-phase extraction has become a critical factor. In recent years, organic polymeric monolith has become very popular for SPE due to its advantages such as easy preparation, high selectivity and high permeability.In this thesis, a porous poly (N-isopropylacrylamide-co-vinyl ester resin)[poly (NIPAAm-co-vinyl ester resin)] monolithic column was synthesized by using N-isopropylacryl amide as monomer; vinyl ester resin as cross linking agent;2,2-azobisisobutyronitrile as initiator; cetyl alcohol as porogen; methanol as solvent. The polymerization was carried out via a free-radical polymerization technique at60℃. The morphology and groups of the materials were assayed by scanning electron microscopy (SEM) and FT-IR spectrum, respectively. The effects of mobile phase flow rate on column back pressure have been investigated. The prepared poly (NIPAAm-co-vinyl ester resin) monolithic column was connected to the HPLC system and used as the adsorbent material for enrichment and analysis of five phenolic compounds in the wastewater. The linearity, precision and recovery have been investigated and verified applicability of the prepared monolithic columns. The experimental results showed that this method has good linearity and precision. The monolithic column is used as the adsorption material for enrichment of phenolic compounds. |
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