Identification Of Dominant Physicochemical Properties Of NPs Involved In Cell Interactions

As the development of nanotechnology, the nature of nanomaterials enable their potential applications in many fields especially in biomedicine fields. Nanomaterials can be regulated as divers sizes, shapes, surface properties etc. in different uses. And the current studies of biological effects of nanomaterials are very limited. The most important biological effect of nanomaterials is their interaction with cells. The cellular uptake of nanomaterials is basic for many other biological effects, so it is crucial to investigate the cellular uptake of nanomaterials.Both macrophages and non-macrophages of Mamalian cells are abled to internalize nanomaterials and show different biological effects. The cellular uptake of nanomaterials can be regulated by change the properties of them. Nanomaterials are hoped to be more internalized by cells when they are used in biomedicine such as drug-loading or image. And nanomaterials which may be harmful to health are hoped to be less internalized by cells. The factors affect the cellular uptake of nanomaterials include size, shape, compsition and surface properties etc. And the different interaction forces between nanoparticles and cells such as electrostatic interaction, hydrophobic interaction, hydrogen bond interaction, π-π interaction and steric hindrance depend on the surface properties of nanoparticles.Now we investigated the interaction between the surface properties of nanomaterials and the cellular uptake of nanomaterials. At the first part we synthesis a library of GNPs with continuous changed surface hydrogen-bond density properties. We connected our ligands with GNPs by Au-S covalent bond, and changed the ratio of different ligands to get GNPs with continuous changed properties. At the second part studied the five libraries of gold nanoparticles (GNPs) in our lab. These five libraries had series of GNPs with continuous changed properties (hydrophobicity, surface charge density, surface hydrogen-bond density, surface π-bond density and surface topology changed). Two cell lines were chosen as our models of macrophages (THP-1) and non-macrophaes(A549). We compared the cellular uptake of GNPs by THP-1and A549in library and inter-library by inductively coupled plasma mass spectrometry (ICP-MS).Result:The surface properties to effect the cellular uptake of GNPs were hydrophobicity, surface charge density and surface hydrogen-bond donor density. The most important factor was hydrophobicity and then hydrogen-bond density for macrophages while surface charge density for non-macrophages..