| Histone code hypothesis was proposed by Strahl and Allis in2000. Multiple histone modifications act in a combinatiorial patterns to specify distinct chromatin states and are heritable. Based on this theory, we applied a scalable subspace clustering algorithm, coherent and shifted bicluster identification (CoSBI), to analyze the distribution features of histone modifications in Drosophila melanogaster genome, in order to study the regulatory models of histone modifications.The purpose of this algorithm is to exhaustively identify the combinatorial patterns of histone modifications based on a given epigenome. We downloaded22chromatin histone modifications and located the coordinate of histone modifications on the genome. Considering the distribution of22histone modifications on the genome, we found out some combinatorial patterns between histone modifications with each biclusters, and we discovered significant correlation feature in all kinds of histone modifications on the whole genome. Furthermore, we identified biclusters with RNA polymerase II and insulators binding proteins. Finally, we applied the algorithm on two groups with different activity (expressed genes and not expressed genes) respectively.In conclusion, we analyzed the combinational patterns of histone modifications on whole genome and functional DNA elements using the CoSBI algorithm. This results may be able to provide an useful clue to understand the regulatory role of histone modifications in gene expression and disease development, and so on. |
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