| Ocean is the place of origin of life.The hot spot has been focused on more complex marine resources because of the decrease of discovering novel bioactive metebolites from terrestrial organisms.During adapting to oceanic ecosystem, marine fungi as a major component of marine microorganisms, can produce numerous secondary metebolites with unique structures, novel skeletons andvaried bioaetivities.In this paper, the antibacterial activity was used as a target goal of bioassay. We developed and validated a convenient method for high throughput screening (HTS) of anti-MRSA and Aspergillus niger agents employing the dye resazurin as an indicator of cell growth. The parameters of the resazurin screening method were optimized. This method showed good reproducibility and sensitivity. It is easy to use, sensitive to changes in cell numbers, and inexpensive.The methodology can be scaled up to primary HTS assays of a large collection of extracts.Based on the results of high throughput screening,we picked two marine fungi(Engyodontium album Aspergillus flavus) to do the research of its secondary metabolites. The extract of the fungus was purified by column chromatography reverse phase chromatography and preparative HPLC combined with TLC,HPLC to get39compounds. Their structures were established by physicochemical properties and spectral analysis. Up to now,13compounds was identified (compound1～13), compound1was identified as new compound. The concentration is200μg/mL, compound1showed good antibacterial activities to Aspergillus niger, MRSA, Vibrio rotiferianus, Vibrio campbellii, Vibrio vulnificus.The MIC was128μg/mL-256μg/mL. Other compounds also shows good antibacterial activitie:compound2,3shows good antibacterial activities to Aspergillus niger, Vibrio rotiferianus, Vibrio campbellii, compound6shows good antibacterial activities to Aspergillus rager,compound10,13showed good antibacterial activities to Aspergillus niger, MRSA,Vibrio rotiferianus, Vibrio campbellii. |
