Investigation Of Neuron Ca²⁺ Response Dynamics With Optical Technology

Rat dorsal root ganglia(DRG) neurons ware successfully cultured and identified, and the calcium ion in vivo cultured neurons was took as the core study in this thesis. The calcium signal in neurons was determined with micro-boards fluorescence detection technology and confocal laser microscope imaging technology, and the concentration of calcium in DRG neurons was quantitatively analyzed. The dynamic response of calcium in DRG neurons which was stimulated by adenosine or triphosadenine(ATP) was tested by optical imaging technology, and the adenosine and ATP were something related to pain.As a result, the vivo cultured dorsal root ganglia neurons were in stable experimental conditions and repeatability, which provided important cell model for studying physiological characteristics of nerve cells. Under the stimulation of adenosine, the calcuim fluorescence intensity in DRG neurons was rising slowly, which was related to the fact that calcium ions flowed into DRG neurons from outside, and also related to the fact that the intracellular calcium stores released calcium ions which were activated by adenosine A1receptor. The dynamic response of calcium in DRG neurons stimulated by ATP was investigated by confocal laser scanning microscopy, and it was found that the calcuim fluorescence intensity in DRG neurons was rising transiently. The transient rising was inhibited by adenosine which pretreated DRG neurons, and it was found that the adenosine A1receptor took part in the inhibition. The results provided important reference value for further studying the specific physiological functions of calcium in neurons and the mechanism of adenosine relief pain.