Font Size: a A A

Regulation And Mechanism Of Led Light Quality In Plant Tissue Culture And Sprouts Cultivation

Posted on:2014-03-23Degree:MasterType:Thesis
Country:ChinaCandidate:L TangFull Text:PDF
GTID:2250330428459682Subject:Botany
Abstract/Summary:PDF Full Text Request
Light quality plays an important role in plant development, morphogenesis, photosynthesis, metabolism etc. Application of different light spectrum of LED is important for plant tissue culture and sprouts cultivation. In this study, callus and plantlets of Anthurium andraeanum were tested to investigate the effects of light quality on adventitious buds differentiation and transplanting plantlets growth, beside Medicago sativa and Toona sinensis seeds were used to investigate the effects of light quality on growth, development, metabolism and nutritional quality of sprouts. Research results will make positive contribution for application for light quality in seedlings industry of A. andraeanum and sprouts industry. The results are as follows:1、The adventitious buds differentiation rate and antioxidant enzymes activity of A. andraeanum callus under different light qualities in vitro were measured. Blue and Red+Blue LEDs were suitable for adventitious buds differentiation, and the rates of adventitious buds differentiation of callus reached100%on28days. Red LEDs, blue LEDs and R/B (1:1) LEDs were suitable for the growth of adventitious buds. SOD activities were significantly lower under blue LEDs and R/B (1:1) LEDs than under other treatments on14and28days respectively. After14days of culture, POD activity was significantly higher under blue LEDs and R/B (1:1) LEDs. CAT activity was significantly higher under R/B (1:1) LEDs on28days.2、The effects of light quality on growth and photosynthesis of transplanting plantlets of A. andraeanum were tested in this experiment. The results indicated that compared with the control and other LED treatments, red/blue (7:1) LEDs significantly increased root activity and chlorophyll a, total chlorophyll and carotenoid content of transplanting plantlets of A. andraeanum; red/blue (2:1), red/blue (7:1) and red/far-red (1:2) LEDs significantly increased the content of soluble sugar, and red/far-red (1:2)LEDs significantly increased the content of free amino acid; red/blue (7:1) and red/blue (2:1) significantly inhabited the content of MDA of transplanting plantlets. As compared with other LED treatments, red/blue (7:1) LEDs significantly promoted the leaf area and root length of transplanting plantlets; Blue, red/blue (1:2) and red/blue (7:1) significantly increased the values of Fv/Fm, Fv/Fo, Fv’/Fm’, ΦPSII, ETR and qP of transplanting plantlets; red/blue (7:1) and red/blue (2:1) LEDs significantly increased the dry mass of shoot and total of transplanting plantlets. These results suggested red/blue (7:1) LEDs promoted the quality and transplanting survival of plantlets of A. andraeanum.3、The effects of different light spectrum distribution on growth, quality and antioxidant activity of alfalfa (Medicago sativa Linn.) sprouts were studied. Red LEDs were suitable for increasing fresh mass of alfalfa sprouts, while dry mass of sprouts was the highest under white LEDs. Blue LEDs significantly promoted the content of soluble protein, free amino acid, Vc content, total phenolics (TP), total flavonoid (TF) content accumulation and radical scavenging activity of DPPH of sprouts, while markedly decreased content of nitrate of sprouts. The content of carotenoid was the highest under white LEDs. The content of quercetin was positively correlated with the activities of PAL in sprouts during growth stage under yellow LEDs. These results suggested that use of blue LEDs could improve partial nutrient quality of alfalfa sprouts.4, The effects of different light spectrum distribution on growth and nutrient quality of Toona sinensis of sprouts were investigated. White LEDs significantly increased edible dry mass, total dry mass, edible rate, and the content of vitamin C and soluble sugar Toona sinensis sprouts. Blue LEDs significantly increased the content of soluble protein and amino acid. Red LEDs significantly reduced the content of nitrate, followed by white and blue LEDs. White and yellow LEDs significantly increased the content of total flavonoid (TF) of sprouts shoot, and white LEDs were also suitable for TFC of sprouts root. As compared with control, red LEDs significantly promoted the content of anthocyanin of sprouts leaves accumulation, followed by yellow LEDs. Overall, white LEDs could benefit the growth of Toona sinensis sprouts, increase the edible rate and improve partial nutrient quality.
Keywords/Search Tags:light quality, light emitting diode, tissue culture, sprouts, cultivation
PDF Full Text Request
Related items