Study On Microbial Communities Influence Production And Oxidation Of Methan In Liaohe Delta

The wetland of Liaohe Delta has many functions, such as water purification, regulating climate, preventing saltwater intrusion on land, and extremely rich in biological resources.We can learn more about the possible contribution of the wetland ecosystem to the atmospheric carbon cycle through studying the influencing factors of methane production and oxidation,and it can provide a scientific basis for reducting the emission of methan.To study the microbial community composition and dynamic variation of Liaohe Delta covered different vegetation,we can assess the status of the soil by microorganisms as an indicator,which is very important to reveal the changes of wetland soil properties.This article taking soil samples of rice,reed and suaeda three habitats of different depths in Liaohe Delta wetland for the study, using methane and carbon dioxide content in the headspace of gas chromatography detection system by building anaerobic methane-producing and methane aerobic oxidation "microcosm",and then study the ability of methane production and oxidation in the wetland covered different vegetation.The study shows that three habitats soil all have not the ability of the methanogenic,but have the ability of methane oxidation.Terminal restriction fragment length polymorphism combine with the physical and chemical properties of different sampling soils was conducted to assess the influence of microbial community dynamics change of Methane production and oxidation by different factors.The study shows that microbial communities of suaeda soil have significant differences with Reed and rice (P<0.05),which maybe because of suaeda in the coastal zone that leading to its communities and inland communities differences;bacteria and archaea T-RF groups of different depth soil have statistically significant difference (P<0.05),which reason maybe is that the pH value and oxygen content is different with the depth increases. In addition, methanogenic archaea was found in the community of the methanogenic system, but produced no methane in the soil, likely is the result of the absence of methane produced precursors(acetic acid).The emergence of methanotrophs verify the conclusions that three habitats are all methane sinks.