The Cluture Method Of Dunaliella Salina In Iodine Rich Medium And Its Biological Function

For human, iodine is essential to health and forms an important constituent of the thyroid hormones. These hormones play a fundamental biological role in controlling growth and development. Iodine deficiency disorders (IDD) has become an global sanitation problem. Inorganic iodine could be dangerous to human and even produces toxicity. Iodine will have wide edible valuation only when it was transformed into biological active organic iodine.With Dunaliella salina chosen as research objects, the growth rates of iodine-rich D. salina were investigated on different methods (KI and KIO3). Secondly, the experiments were designed to measure the iodine enrichments of D. salina in iodine-rich medium. Finally, the changes of quality were studied on D. salina in different concentrations of KIO3.The conclusions obtained were stated as follows:KIO3, compared with KI, was an optimal iodide for accumulation of iodide in D. salina cells and its appropriate concentration for culture of iodine-rich D. salina was0.5g/L. Under this concentration, the dry weight harvest of D. salina experimental group (545mg/L) was as about1.52times as the control (384mg/L). Algal cells resulted in highest accumulation rate of iodine that the total iodine content in experimental group (110.48mg/kg) was as about5.3times as the control (110.48mg/kg). Inorganic iodine content of experimental group (10.39mg/kg) was about1.30times as the control (7.97mg/kg) and organic iodine (100.09mg/kg) was as about8.43times as the control (11.87mg/kg). The organic iodine accounted for90.6%of total iodine content in cells of D. salina. The content of protein in D. salina experimental group (432.51mg/L) was increased about6%as the control (409.03mg/L). The content of chlorophyll a in D. salina experimental group (8.939mg/L) was increased about65%as the control (5.724mg/L).The content of β-carotene in D. salina experimental group (9.08mg/L) was increased about48%as the control (6.12mg/L). A comparative study between continuous aerobic incubation and shaking flask culture showed that contribute to the increase in these indicators by single factor, and had the feasibility of the production. The continuous aerobic incubation experimental group was benefited for the detection indexes in D. salina.This paper studied on the scavenging effects of iodine-rich D. salina and D. salina ethanol extract on super oxide anion and hydroxyl radical. Capabilities of scavenging for OH· and02-· by ethanol extracts from iodine-rich D. salina and D. salina were determined by the inhibition of pyrogallol auto-oxidation and monitoring the bleaching of safranine T by hydroxyl radical generated from the Fenton reaction in vitro, respectively. The results indicated that the rate of iodine-rich D, salina ethanol extract was higher. The clear trend was similar, but active of super oxide anion is different. And the antioxidant activity of iodine-rich D. salina ethanol extract was much higher than D. salina ethanol extract by detecting the content of MDA.In conclusion, D. Salina was absorbed iodine apparently, and it was more feasible to the scavenging effects on free radical and the quality with iodine-rich D. salina. So it was of promising market applications to cultivate iodine-rich D. Salina for biofortification.