The Improvement Of The Promoter Cbh1and The Construction Of Heterologous Gene Expression Multi-purpose Vector Of Trichoderma Reesei

The Trichoderma reesei is an important filamentous fungus in the industrial fermentation and gets more and more attention in recent years because it can express and secrete exogenous protein strongly. The reesei has been applied for production of cellulase for many decades. With the protein modification capabilities, the reesei is considered about its potentiality to produce mammalian proteins. However, the use of filamentous fungi as hosts to express foreign proteins, especially non-filamentous fungal protein, its secretion efficiency is not good enough. The study was to construct several efficient exogenous expression vector for Trichoderma reesei.The cbhl promoter has been considered one of the strongest promoters in T.reesei. While according to the report, there are several potential glucose repressor binding sites on the strong promoter CBHI. Our study was to improve the promoter CBHI to improve the expression efficiency of exogenous gene and to construct a significant vector using the promoter and terminator of CBHI from Trichoderma reesei. The results showed that the reform of the promoter CBHI improve the expression level of exogenous.We conducted the present study to construct four binary fungal expression vectors, all with the strong fungal promoter of CBHI. The vectors Kg and Kh, can be used for random integration, and the other two, Kg and Kh, allows for site-directed integration. The results also showed great prospect of the using the new constructed heterologous gene expression multi-purpose vector to level up the production ability. And the genetically modified strategy of Trichoderma reesei will gain more attention.