Construction Of Magnetic Carbon Carrier And Its Influence On Microbial Activity

Magnetic carbon carrier is Fe3O4nanomaterials as nuclear, inorganic carbon as a shell coated thereon, so that the product of the combination of magnetic nanotechnology Fe3O4nanomaterials become compatible with the biological activity.The magnetic carbon carrier can be used as a drug carrier in biomedical pathway through the vein, administered orally or injected directly under the external magnetic field boot.Drug selectively reach the target releasing to play the best efficacy. Magnetic carbon carrier can be used in the immobilized enzyme. It is possible to control the movement and orientation of the immobilized enzyme in the magnetic field effect, greatly improving the catalytic efficiency of the enzyme, and it is conducive to the recycling of the enzyme, the enzyme can be repeated use, the costs greatly is reduced. Combined of magnetic carbon carrier with excellent value, thesis mainly makes following researches.(1) In the hydro-thermal method the Fe3O4nanoparticles of the product are prepared and by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) transmission Electron Microscopy (TEM), and the superconducting quantum interference device (SQUID),the structure, magnetic properties and morphology and of Fe3O4nanoparticles are characterized. The results showed that the Fe3O4nanoparticles prepared by FeC2O4are ineffective. Two kinds of Fe3O4nanoparticles prepared by FeCL2smaller were10-20nm and50nm, superparamagnetic. Two kinds of Fe3O4nanoparticles materials are selected to do antibacterial experiments. With hydrazine hydrate as the precipitation of Fe3O4nanometer material prepared, when the concentration are7mg/mL and9mg/mL, the inhibition rate can reach100%to Bacillus licheniformis and Escherichia coli. With ammonia as precipitant for preparation of Fe3O4nanometer materials when the concentration are5mg/mL and7.5mg/mL the inhibition rate can reach to Bacillus cereus and Serratia marcescens.These two the Fe3O4has good antibacterial effect. Further illustrate the necessity of the inorganic carbon coated.(2) The iron acetylacetonate ferrocene iron source, glucose, sucrose as carbon source and ammonia as the precipitating agent, hydrothermal synthesis of magnetic carbon carrier and the structures, morphologies and magnetic properties of the products are characterized by X-ray powder Diffractometer (XRD), Scanning Electron Microscopy (SEM) and Superconducting Quantum Interference Device (SQUID), respectively. XRD patterns of the test results showed that the nanoparticles prepared with standard Fe3O4nanoparticles largely consistent, the internal ofmagnetic carbon carrier is Fe3O4, most of magnetic carbon carrier size less than100nm.IR test shows magnetic carbon carrier have a large number of the secondary amine group, a carbonyl group, a hydroxyl group, an amide group, the polar group. These groups can be connected with biomolecules. The magnetic carbon carrier has a wide range of applications in the biological field. The magnetic carbon carrier(the5samples), by2mol iron acetylacetonate and2mol glucose preparation,are uniform size, the particle size is30nm, saturation magnetization is81.3emu/g, the coercivity is4.90Oe,the remanent magnetization is6.25emu/g, The magnetic carbon carrier is superparamagnetic. The XRD diagram show that this carbon coating effect is best.(3) No.5-8, samples prepared are made antacid experiment.Antacid result shows No.5sample is best.lt proved No.5samples covering effect. No.5sample past7hours in a1mol/L hydrochloric acid,with completely no discoloration, and the antacid effect is superior with having a magnetic. And No.5sample has a broad application prospects.(4) Magnetic carbon carrier has good compatibility with microorganisms.After magnetic carbon carrier is added to the medium, the growth of microorganisms completely not subject to any inhibition.The prepared magnetic carbon carrier can be widely applied in the biological. A stability test on the material show that material can be repeatedly used five times without any inhibition of biological.The test of magnetic carbon carrier on microbial enzyme production show that enzyme activities doesn’t completely suppressed.By doing this experiment we also found that the magnetic carbon carrier has the enzyme activity, and it has relatively high enzyme activity.(5) Research magnetic carbon carrier in different concentrations, pH,time, temperature, and sorption rate of enzyme, the conclusion is15mg/mL,pH7,Temperature20℃,adsorption90min,adsorption reached maximum0.03ug, accounting for33%of the amount of1mL enzyme solution containing enzyme.Adsorption efficiency is better. In optimal conditions for adsorption of phospholipase Al, magnetic carbon carrier measured enzyme activity, its activity was found to15U/mL higher than the enzyme activity of the enzyme solution25%. Magnetic carbon adsorption phospholipase Al carrier stability test results show that it is continuous use three times,the enzyme activity are relatively stable, with an attractive prospect.