| The study using Escherichia coli and its multiple related H2O2Gene mutants asmaterials, using visible and ultraviolet spectrophotometry、transmission electronmicroscopy techniques, research H2O2mediated function in mesosomes of bacteriaand its molecular mechanism; investigate the functions of mesosomes that H2O2participate in cell division; clarify problems about additional generation of endogenousH2O2and the changes of spatio-temporal localization, and analysis these additionalH2O2produced intracellular sources. Completion of the project will further clarify andimprove mechanism of action and biological function of H2O2. The interpretation forcompleted mechanism of the specific cells-mesosomes function in bacteria will beusefull to further in-depth study functional of bacteria.The experimental results show that: Adjust and improve electron microscopystaining method on the basis of conventional histochemical methods, ensuring thatH2O2staining and the sample fixed at the same time is a desirable method ofpositioning endogenous H2O2of the bacterial cells.Determine the temporal and spatiallocalization of E. coli H2O2in the cell division cycle. Interphase of cell division, Genemutations and exogenous CAT processing can affect the level of intracellular hydrogenperoxide. Having a lot of H2O2production and accumulation identified thatmesosomes are the novel source of extracellular H2O2production in vitro. Mesosomecultures involving hydroxylamine hydrochloride treated by ice bath for10min at4℃and warm bath at40℃for40min, are the best conditions for re-release of H2O2.Mesosome plays a positive role for the protoplast regeneration. Conclusion: Additionallocations of the accumulated H2O2are closely associated with the process of celldivision of Escherichia coli strains investigated. Spatio-temporal localization changesof endogenous H2O2should be an ubiquitous phenomenon during cell division cycle ofbacterium. H2O2plays key roles in bacterial proliferation. |
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