The Effect Of Bisphenol A On Expression Of Related Genes In Reproductive System Of Neonatal Mice

Environmental hormones is one of the hot spots in the modern life scienceresearch. Nowadays more and more people begin to pay attention to this topic. It isfilled with a variety of chemical toxic reagents in our living environment. Amongthem, there’s a class of substances which can simulate or inhibit hormone activity,called endocrine disruptors. Endocrine disruptors have the ability to change thestructure and function of the endocrine system. Bisphenol A is an environmentalestrogen, belong to one of endocrine disruptors. Bisphenol A is widely used in themanufacture of polycarbonate plastic and epoxy resins. Bisphenol A has a weakestrogenic effect and can bind to estrogen receptors, causing the endocrine systemresponse. The present study shows that bisphenol A affect embryonic developmentcross the blood placental barrier and damage the neuroendocrine system, liverfunction and reproductive development.This topic is mainly to inject the newborn mice with bisphenol A, observingweight change for4weeks after injection and weight of main organs at4weeks old.We pay more attention to the statistical analysis and morphological observation ofgenitals. We also make the quantitative analysis for the expression of related genes inreproductive system and validate it by in situ hybridization on tissue.After injection, weight of mice hasn’t significant difference, but bisphenol Aaffects weight of some organs. At genital aspects, bisphenol A changes weight ofovary but not that of testis. The wall thickness of seminiferous tubule and the numberand form of follicle in ovary are no abnormal. Bisphenol A of different doses maycause up or down regulation of gene expression inordinately, of course, some ofinfluences will change depend on the sex.