MMARVELD1Expression In ES Cell Differentiation And Its Influence On EBs

Embryonic stem cells are a class of pluripotent cells in vitro environment andcan be induced to differentiate into almost all living tissues and organs. EScells,therefore,become the ideal materials of stydy biology development and geneticoperation. It is also known that embryoid bodies with spherical structure can begained from ES cells spontaneously differentiation when cultured in the mediumwithout LIF, and it can exhibit a certain degree of early stage developmentalembryological characteristics, therefore, the gain and research of embryoid bodyhave become an important means to study biological development.To understand the function of MARVELD1, we divided the research into twoparts:the expression changes of murine MARVELD1gene in the formation progressof embryoid body from ESC differentiation, and the analysis of the gene effect onembryoid body formed. This study has laid an important foundation for explorer thebiological effects of mMARVELD1during embryonic development.This paper explored the changes of the mMARVELD1gene expression in theprocess of the ES cells and its differentiate to form embryoid bodies. Theexperiments using mouse embryonic stem cell line ES R1as experimental materials,and it cultured in LIF dislodge into embryoid bodies (EBs). Subsequently,weexamined the mMARVELD1expression changes of the ES cells differentiate to EBsformation. Further, we analysis of the gene whether or not it have an important roleduring early development. Study found that, mMARVELD1gene low expression inES cells, and the gene expression level in general showed an increasing trend withthe differentiation of ES cells, especially in the ES cells formed into embryoid bodyof8d, the gene expression at the highest level, indicating that the gene may beinvolved in the differentiation of ES cells and its formation to EBs process.Then, to study the effect of mMARVELD1on the progress of ES cellsdifferentiated into EB, the research also silence the gene by RNAi, and then observethe morphological dirrerences in the formation process of embryoid biodies from EScells with low mMARVELD1gene expression. At the same time, the research hasconstructed mMARVELD1gene knockout vector by using further molecular cloningtechnology and method, and used the vector as experimental material to grope thecondition of electricity transfection for ES cells, in order to obtain mMARVELD1loss genotype ES cell clones, further to lay a foundation for deply explorer thebiological function in the early development stage.