Damage Effect Of Nano-ZnO On Gene Change Of Liver Tiusse Of Zebrafish(Danio Rerio)

Zinc oxide (ZnO) nanoparticles have very small size and particular properties. With the large-scaleproduction and wide applictation in all fields, the bio-safety of manufactured nanomaterials and nanotechnology,as well as the hazards of humen health and environment has attracted extensive worldwide attention. In therencent years, some reports provided the principle mechanism about the oxidative stress and ROS, and a littleabout the gene effect, such as DNA damage. However, the molecular view of their toxicity on aquatic organism isscarce, especially on liver tissue of Danio rerio. In the current work, we use liver tissue of Danio rerio as the testorganism and target organs, use paraffin section, Phytophysiology and Biochemistry, RT-PCR, qPCR, as well asDGE, research the damage effect of nano-ZnO on zebrafish liver. The aims were to know the molecularmechanism of nano-ZnO on the aquatic organism, further more, and provide the basis of its biological effect onenvironment.Nano-ZnO was measured by AFM; the particles were pure, round and the primary sizes were20±10nm.Damage phenomenon was observed at more than5.6mg·l-1nano-ZnO by paraffin section. After explorednano-ZnO at different concentration (2.8mg·l-1,5.6mg·l-1,11.2mg·l-1,22.4mg·l-1,44.8mg·l-1), compared withcontrol group, the GSH level was significantly decreased (p<0.05); MDA level increased with concentrationsignificantly(p<0.05); Na+K+-ATPase activity decreased first and then increased (p<0.05)with the time change;ROS level was higher than control for24h. It is indicate that oxidative amage was found in Danio rerio whenexposed nano-ZnO. The generation of oxidative stress, disrupted the balance of oxidoreductase system, and thenliver tiessue was damaged. The RT-PCR result show that at least four oxidative stress gene mRNA of Danio reriowere significantly changed after exposed nano-ZnO, include peroxiredoxin gene(prdx), catalase gene(cat),Gu/Znsuperoxide dismutase gene(Gu/Znsod) and glutathione peroxidase4gene(gpx4). Compared with the control, therelative expression levels were first increased and then decreased. It demonstrated that the nano-ZnO influence theoxidative stress gene mRNA expression level of Danio rerio mRNA. Digital gene expression (DGE) analyseddifferentially expressed gene cluster, GO and Pathway, revealed that compared with the control, the differentiallyexpressed gene significantly in organelle membrane, organelle part, envelope and mitochondrial part of GOcomponent, oxidoreductase ativity of GO function, as well as carboxylic acid metabolic process, organic acidmetablic process, cellular ketone metabolic process, small molecule metabolic process and cellular amino acidmetabolic process of GO process. KEGG analyzed, the greatest differentially expressed gene pathway wasoxidative phosphorylation. The conclusion was that, the oxidative stress was the majoy influence of liver tissueand mitochonodria oxidative phosphorylation was one of the important pathways.