The Flow Field Simulation And Analysis Of Shaking Heilical Bioreactor

With the increasing demand for medical protein, culturing animal cell by bioreactorin larger volume has become a trend. Stirred bioreactor has three drawbacks, that isexpensive, larger shear flow field and making studies at small-scale difficult toextrapolate to large-scale, so shaking bioreactor has attracted the attention of scholars.Because shaking bioreactor in Harbin Pharmaceutical Group has low oxygenconcentration and poor mixing result, so the aim of the thesis is to improve originalstructure by adding a circle helical baffle. The internal flow field in shaking bioreactor issimulated by Fluent software, and the results are analyzed.Firstly, the form of liquid movement within a helical track into a cylindrical vessel,square-shaped, cylindrical shaking bioreactor is simulated by Fluent software, the resultsshow that the liquid in helical shaking bioreactor moves along the helical track, the liquidin cylindrical shaking bioreactor is predictable and scalable, the liquid in square-shapedis the most complicated for the presence of edges and corners.Then the thesis establishes the relationship between evaluation criteria andsimulation results in shaking bioreactor and optimizes width and pitch in helical track,and the result show that the optimal width value is125mm, the optimal pitch value is166mm.Furthermore, considering the effect of spread and the amount of liquid to theinternal flow field in shaking bioreactor, the simulated results show that increasingspread can improve oxygen concentration and mixing result, increasing liquid leads tolow oxygen concentration and poor mixing result so that it may emerge lack of thesupply of sufficient nutrient during the growth of animal cells.Moreover, the internal flow field at different amount of liquid in a helical track intoa cylindrical vessel, square-shaped, cylindrical shaking bioreactor is simulated, and theresults show that a helical track into a cylindrical vessel shaking bioreactor has higheroxygen concentration and better mixing result than the others.Finally, the thesis measures the mixing time in the improved and original structureby experimental method and considers the effect of spread and the amount of liquid tothe mixing time, and the conclusions from the mixing time match the correspondingsimulation results by the mixing time.