| During the experiment，by using tail vein and bottom of mammary glandular injection, thebone marrow mesenchymal stem cells(BMSCs) transplanted to the rats of different growth period,meanwhile influence and mechanism of the BMSCs in rat mammary gland development werepreliminary discussed. Methods:120SD female rats were randomly divided into3groups，twoexperimental groups and one control group,40in each, and weight difference of the rats in groupswas not significant (P>0.05); BMSCs were diluted to the point of1×106with50μL salinesolution and injected to tails vein and bottom of mammary glandular of the two experimentalgroups respectively; with the same method, the control group is injected with the same volume ofsaline solution. When the rats reached their first puberty after20d,60d, and after (Pregnancy18d)80d, BMSCs are transplanted to the test group, control group were also injected with the sameamount of normal saline; when the rats reached their30d,80d,and after (Lactation stage15d)100d,8rats were selected from each group randomly, then their life weight measured before theyare disposed, heart blood serum prepared, and E2, P4, GH, PRL, IGF-1, EGF, TGF-α, AR, TGF-β1,aFGF, bFGF, LEP, HGF, Glu, Alb, TP, TC, TG, ALP and LDH levels are tested in order; Afterdissected, wet weight of uterus, ovarian and mammary gland were measured; when they reachedL4d period, six nests were selected for each group,8cabs of rats remain in each nest, whichmeans each lactation rat with eight cubs, and then rat lactation volume experiment was carried out.From the experiment, the following results are clearly shown:First, after BMSCs transplantation to the rats of different growth and development stages, E2,P4and IGF-1level in the serum of rat80d of experimental group are significantly higher than thatof control group (P<0.05), at the same time the body weight of rats and uterus weightsignificantly higher than that of control group (P<0.05), differences of those in30d and100dwere not significant (P>0.05); Throughout the trial, GH levels in the serum and ovarianrestructuring differences were not significant (P>0.05) between two group. Second, after BMSCstransplantation to rats in different periods, change level of PRL, EGF, TGF-alpha, AR, TGF-beta1,aFGF, bFGF, LEP and HGF in the serum were different. Experimental rat80d serum PRL, EGF,TGF-alpha and bFGF were significantly higher than that of control group (P<0.05),30d and100d differences were not significant (P>0.05); During the whole process, change of the AR, TGFbeta1, aFGF, and LEP level in the serum of rats were not significant (P>0.05). Third, weight ofthe rat80d mammary gland significantly higher than that of control group (P<0.05), and30and100d differences were not significant (P>0.05). Forth, mammary gland bubble number, itsChamber capacity diameter, and area of rat80d and100d of experimental group are significantlyhigher than control group (P<0.05), the experimental group’s rats L10d and L12d mammaryvolume are significantly higher than control group (P<0.05). Fifth, after BMSCs transplantation,change level of the Glu, TP, TC, TG, ALP, LDH in serum are changed, TP and ALP level in serumof80d are plainly higher than control group (P<0.05), change level of30d and100d were not significant (P>0.05); In the whole process, change level of Glu, TC, TG and LDH in serum of ratsin the test group and control group were not significant (P>0.05). Sixth, during the whole test,4kinds of hormones such as estrogen and IGF-1,9kinds of cytokines and7kinds of bloodbiochemical index levels in serum of rats in the bottom of mammary gland injection transplantedgroup is slightly higher than the tail intravenous injection transplanted group, but the differenceswere not significant (P>0.05). Mammary gland injection group L12d had a significantly higherLactation volume than that of tail intravenous injection group,(P<0.05).Finally, by using tail vein injection and Mammary gland injection to transplant BMSCs todifferent growth pubertal rats, affected the mother in late pregnancy serum IGF-1, EGF, TGF-alpha, AR, TGF-beta1, aFGF, bFGF, LEP and HGF levels; through the endocrine and paracrineregulation, this changed the pregnant rats’ serum Glu, Alb, TP and TC, TG, ALP, LDH level,increased the body metabolism level, thereby promoted pregnancy rats’ uterus and breast weightgain, promoted breast tissue morphological development, in turn, this also increase the amount oflactation. Among these two approaches of BMSCs transplantations, the effect promoting themammary gland of the mammary gland base injected BMSCs transplantation is better thanintravenous transplantation method. |
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