Metabolomic Analysis Of Thellungiella Salsuginea Under Salt Stress Via~1H-NMR Spectroscopy

1H-NMR-based metabolic profiling approach was used to identify metabolome changes in rosettes of Thellungiella salsuginea under NaCl stress in an attempt to understand the mechanism of salt tolerance. The metabolic network was constructed by detecting and analyzing the majority metabolites. Metabolomic analyses showed the existence of several physiological strategies for tolerance of salt stress in shoots of T.salsuginea. In out experiment, we detected23small molecular weight intracellular metabolites from the rosette of T.salsuginea, comprising11amino acids,4sugars,6organic acids and two quaternary ammonium compounds. Principal components analysis (PCA) indicated highly significant separations (p<0.05) between control and NaCl treatment, and the150mmol/L and300mmol/L NaCl treatment also impacted the metabolome of T.salsuginea differently. Forthermore, the metabolomic data was analysised by using cluster and correlation analysis. The results indicated the varied responsive model and complicated relationship among the different metabolites in response to salt stress.Based on the metabolomic data of quantitative determination, we profiled the matebolic pathway of the majority metabolites and mapped the metabolic network by appling VANTED. The metabolites we detecting involved in carbohydrate metabolism, amino acid metabolism, TCA cycle and betaine metabolism. The content changes of majority metabolites combined with the functional analysis of metabolites explained the roles of each metabolite in osmotic regulatory, stress defense and energy metabolism adjustment. All the information help us to understood the metabolic responsive strategies of T.salsuginea, which included expectedly accumulating osmolytes, reducing oxidative stress during whole process and increasing energy donator with short-cutting the energy metabolic pathway.