The Screen And Study Of PRMT-6Regulating Aging By High-throughput96-well RNAi System In C.elegans

It has been known that protein post-translational modifications play important roles in aging regulation. However, there are still limited genes about these modifications that have been reported to function in aging. One of the biggest problems in this research field is the lack of a high throughput screening system for aging study. Caenorhabditis elegans is one of the important models for study aging because of its short life cycle. In addition, the simple RNAi screening system has been builded and used broadly in this roundworm for many years. Therefore, it is very necessary to establish a high throughput RNAi screening system to investigate genes involved in aging regulation in C. elegans.In this study, we established a new96-well high-throughput RNAi screening system in C. elegans based on the methods of96-well liquid worm culture, RNAi screening and life span assay. PRMT-6, one member of arginine methyltransferase family, was screened for aging regulation through this system from the RNAi library. This library includes115bacteria strains which expressed the dsRNAs of genes participated in protein post-translational modifications in C. elegans. Furthermore, our results of knockdown prmt-6in daf-2, eat-2, clk-1and glp-1mutant worms, showed that PRMT-6regulated aging by Insulin/IGF-1singling pathway (IIS), but not by energy limit signaling pathways, mitochondrial respiratory chain/ATP synthesis system signaling pathways, and gonad related signal pathways. Our results then indicated that the function of PRMT-6in regulating aging, was dependent on SKN-1, but not the other key factor DAF-16in IIS pathway. These studies will provide a new way to screen and study the genes participated in aging regulation in C. elegans.