The Character Of The Water Resources And The Choose Of Pb-resistance Strains In Nansha River

Nansha River, the branch of Fenhe River, located in west of Jianshe Rd, east of Fenhe River in Taiyuan. With the development of industry, a large number of waste water discharge into Nansha River, which results in deterioration of river and has seriously affected the coastal landscape and the physical and mental health of residents. Therefore, Efforts to solve Nansha River ecological environment are in the urgent. In recent years, the microbial adsorption of heavy metal ions provides a new approach to environmental governance.Firstly, Nansha River was seperated into four sections according to the flow direction. Plate colony-counting method was used to determine the microbial groups of the four section; alkaline potassium pursulfate digestion-UV spectrophotometric method, ammonium molybdate spectrophotometric method and FAAS were carried out to determine the concentration of water pollutants of the four sections.The results showed that, the microorganism quantity of Nansha River was ordered as bacteria> eumycota> actinomycetes; the concentration of pollutants was ordered as TN> TP> Pb> Cr> Cd.Secondly, comprehensive pollution index method was taken to assess the water quality of Nansha River; analyzed the correlations between pollutants (TN, TP, Cd, Cr and Pb) and bacteria, eumycota and actinomycetes. The results showed that, the pollution index was larger than1, which was more than the surface water standards in Taiyuan city, belonging to V-level of national surface water; the pollution load ratio was ordered as TP> Pb> TN> Cr> Cd; the water quality comprehensive index of the four sections (S1, S2, S3and S4) were10.4007,10.9775,10.0878and9.6713, the pollution situation was ordered as S2> S1> S3> S4. Moreover, correlation analysis indicated that significant positive correlations existed among TN, TP (p<0.01) and bacteria, eumycota, whlie significant negative correlations existed among Cd. Cr, Pb (p<0.01) and bacteria, eumycota. The significant negative correlations existed among Cd and bacteria (p<0.01) and eumycota (p<0.05). The significant positive correlations existed among TN (p<0.01) and actinomycetes, whlie significant negative correlations existed among Cd, Cr, Pb (p0.05) and actinomycetes. There were no significant correlations among actinomycetes and TP. There were significant negative correlations between pollutants TN. TP and heavy metal Cd, Cr, Pb.Thirdly, this research isolated the Pb resistance strains from the Nansha River, studied and compared the Pb ions enrichment characteristics of growth bacteria and dry bacteria. The results showed that the adsorption rate of N2bacteria was higher than bacteria N1and bacteria N1, N2were higher than the dry microbial cells. bacteria N2adsorption rate was88.44%, bacteria N1adsorption rate was69.41%, N2dry bacteria adsorption rate was71.23%, N1dry bacteria adsorption rate was53.76%,which maybe resulted from that the characteristics and structures of cells changed by dry and the cell deformation interfered Pd infiltrated into the intercellular area. Therefore, the reason of less Pd accumulation sites and decreased ability of dry bacteria was cell deformation, or ligand on the cell surface reducing. Therefore, Pd adsorption was not only cell surface accumulation but also ions transferred to the interior of cell. Adsorption rate of N1, N2dry bacteria and growth bacteria reached more than50%,which proved that bacteria had high ions adsorption ability.After morphological characteristics and16S rDNA sequence analysis, strain Nl and N2were certified Bacillus cereus. This research provided the experimental and theoretical basis of ecological management scheme of Nansha River.