| The mammalian embryo implantation is an extremely complex process. Theblastocyst invasive and the establishment of endometrial receptivity are keys to ensurethe successful implantation of the embryo. In these processes, the estrogen andprogesterone act on receptors in the Endometrial cells to make interstitial edema,vascular congestion, which provide the material basis for the implantation of theembryo. Endometrial cells secrete large quantities of cytokines, proteolytic enzymesmaterial to promote the mutual recognition, adhesion of the embryo and endometrium.β1,4-galactosyltransferase enzyme I （β1，4-GalT-I） is divided into twophenotypes, which are short-chain and long-chain. Short-chain β1，4-GalT-I,distributed in the Golgi apparatus, exercises the functions of glycosyltransferase.Long-chain β1,4-GalT-I,located located on the cell membrane,acts as a cell adhesionmolecule playing an important role in cell adhesion, sperm-egg recognition, neuritegrowth, tumor migration process, etc.TGF-β1is one of the transforming growth factor-β superfamily members, two-wayfunction to promote and inhibit of cell proliferation, and will display different biologicalroles for the different target cells, as well as different functional states of the same targetcells.Objective:This paper mainly discussed the expression of β1，4-GalT-I aftertreating hormone and the relativity of β1,4-galactosyltransferase I and TGF-β1, andthen explored the regulatory mechanism of embryo implantation.Method:（1）.Estrogen and progesterone, respectively，in accordance with the timegradient and the concentration gradient were added into RL95-2cells （analogimplantation of endometrial cells）to co-culture, using RT-PCR to detect β1，4-GalT-Iexpression.（2） Transfected β1，4-GalT-I gene over-expression plasmids and empty vector plasmids into RL95-2cells and then detected total β1,4-GalT I and long-chainβ1,4-GalT I expression by Western-blotting （3） Transfected β1,4-GalT-I geneover-expression plasmids, interference plasmids, interfere with the control plasmidsand empty vector plasmids into RL95-2cells and then detected total β1,4-GalTI,long-chain β1,4-GalT I expression and TGF-β1expression in each group oftransfected cells by RT-PCR and immunofluorescence.（4） Cultured progesterone andestrogen （40μg/μl） in accordance with RL95-2cells which were divided into normalgroup, control group, estrogen group, the progesterone group and the estrogen withprogesterone group, and detected the change TGF-β1expression by ELISA in eachgroup concluding these of transfected cells.Results:（1） The expression of total β1,4-GalT-I reached the peak in estrogen rolefor the concentration of10-4ug/ul, time for12h（P<0.05）; The expression of total β1,4-GalT-I reached the peak in estrogen role for the concentration of10-4ug/ul, time for12h（P<0.05）.（2） After being transfected by β1，4-GalT-I gene over-expression plasmids,the protein expression of β1，4-GalT-I is increased obviously（P<0.05）（3）. After beingtransfected by the four kinds of plasmids and cultured for24h, the effect of transfectionby the emergence of the green fluorescent protein is well.After RL95-2cells weretreated with β1，4-GalT-I gene over-expression plasmid transfection, the expression ofthe total β1,4-GalT I gene and long-chain β1,4-GalT-I gene were significantlyincreased（P<0.05）; But the expression of the TGF-β1gene was not changed obviously.After RL95-2cells were treated with β1，4-GalT-I gene interference plasmid，theexpression of the total β1，4-GalT-I gene and long-chain β1,4-GalT-I expression weresignificantly decreased（P<0.05）; But the expression of the TGF-β1gene was notchanged obviously.（4）. Treated RL95-2cells by estrogen and progesterone, and thegroups with hormones were significantly higher than the normal group（P<0.05）.But,the group of transfected cells was not changed obviously.Conclusion：（1）Hormones might regulate the implantation of the embryo throughthe β1,4-GalT-I.（2）.β1,4-GalT-I might not affect the expression of TGF-β1.（3）Hormones might regulate the expression of TGF-β1. |
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