Biosystematic Studies Of Curex Pisiformis Complex

This study used cpDNA TrnL-F, atpB-rbcL. RPL16, TrnV and ITS sequence to analyse the phylogeography of Carex pisiformis Complex plants. According to cpDNA sequence variation of121individuals from12populations for genetic diversity and genetic differentiation analysis. We identified the geographical distribution of haplotype patterns and traced the evolution of the modern distribution of population history, historical reasons of distribution pattern and possible glacial refuges. The results are mainly as follows:(1) Four combined sequence length was between3305to3329bp. its average G+C content was33.4%which conform to majority cpDNA intergenic seuuence. and were identified9kinds hapiotypes (h1-h9). Carex pisiformis Complex take on relatively higher haplotype diversity (h=0.894) and nucleotide diversity (π0.07087). Average gene flow (Nm=0.067) was in less frequent.(2) Analysis of molecular variance (AMOVA) results indicated that genetic variation mainly existed among species (98.93%). and genetic differentiation had a high degree between populations (Fst=0.94332. Gst=0.457,Nst=0.827). Nst＞Gst (p＜0.05). phylogeographic structure is clear.(3) Mantel statistics result (r=0.3742. p＜0.001) indicated that geographic distance between populations and geographic distance were positive correlation. All three kinds of neutral test value were negative (Tajima’s D=-0.09069; Fu and Li’s D*=-0.12389; Fu and Li’s F*=-0.13139) and not significant (P＞0.10). Thus gene locus evolution pattern does not reject neutral hypothesis.(4) Speculating glacial refugia of Carex pisiformis Complex are south Zhejiang district and Mt. Tsinling area. According to NCA result inferred that haplotype geographical distribution pattern is mainly caused of allopatric fragmentation and restricted gene flow with isolation by distance etc.(5) C. hypochlora and C. pisiformis are original group of Carex pisiformis Complex, their genetic variation are not high. C. hreviarislata. C. dapanshanica and C. pseudotristachya have closed evolutionary relationships. C. daividii is a relatively independent species in Carex pisiformis Complex.C. subebracteata had a certain extent differentiation which may be cause by the narrow geographical environment of Inner Mongolia.(6) Linking all molecular results, admit all the9taxa involved in this study are recognized, including new published taxon Carex dapanshanica.