Microalgae Fast Screening And Laege Scale Cultivation For Bioenergy Utilization

An integrated methodology from micro-scale strains screening to large-scalebiomass production of microalgal bioenergy was built up in this study. A96-well microplates swivel system （M96SS） was designed for microalgalstrains screening for bioenergy utilization. The concordance of M96SS onstrains screening versus traditional standard flasks culture screening wasverified. Critical problems of intra-well mixing and evaporation loss wereimproved by sealed vertical mixing of the M96SS. The reliability andefficiency of the M96SS were verified through a comparison of traditionalflask culture and previous microalgal miniature culture methods. During thescreening of6microalgal strains, Chlorella sp. SJTU-3was selected asresearch material for further large-scale biomass production because of itshigh biomass yield in artificial medium (1.229g L^-1) and also goodperformance in wastewater medium under20%CO₂. Illumination wasdetermined as the main limitation factor for the raise of biomass yield ofChlorella sp. SJTU-3in its small-scale cultivation （500mL） in air-lift columnphotobioreactors. Finally, based on the data of preculture,9000lux double-side illumination and10%CO₂concentration were selected asoperation conditions for culture optimization in pilot-scale （160L） tubularphotobioreactor. The modified biomass yield （7days culture） was1.05g L^-1,which was2.5times higher than that in preculture.