Design Of Portable Flow Cytometry Based On ARM

Flow cytometer (FCM) is a collection of electronic and physical technology, photoelectric measuring laser technology, computer technology and the cell fluorescence chemical technology, monoclonal antibody technology as a new type of high-tech instruments. Since the first flow cytometry instrument was developed in1953, flow cytometry instrument has obtained the development by leaps and bounds. Flow cytometry instrument as a whole is divided into two kinds, namely several and clinical type. Development of portable flow cytometry instrument as a clinical application is a big hot spot in the field today. At home and abroad for the development of portable flow cytometry instrument are still in the laboratory stage, after having a certain understanding of the related background of flow cytometer instrument, this thesis come up with a general idea that of a set of portable flow cytometry instrument controlled by ARM microcontroller. The instrument consist of the following parts:The micro-fluid chip platform; Optics inspection system; ARM-controlling system; Data acquisition and storage system; Digital signal processing system; Power supply system, etc. For time is limited, this thesis mainly accomplish part of this system, namely the ARM-controlling system and data acquisition system. That specifically means:(1) Build the optical detection system used to detect the signal of fluorescent particles, the optical source is a blue LED with a center wavelength of488nm, in addition, the optical detection system also contains optical devices such as filter, photodiode, etc.(2) Rewrite driver program on OK6410development board built-in ADC chip, using the built-in ADC to acquire external analog input signals, and with QT, a GUI development software, we write pulse signal counting program based on threshold value method and downloaded it to the ARM embedded development board for running, accomplishing the building of the whole data acquisition and analyzing system.(3) Using ARM embedded development board GPIO pin to simulate SPI bus to control external ADC chip, and use AD7707chip to replace the built-in ADC chip in the data acquisition and analyzing system. Accomplishing the acquisition of the signal being dealt with the PD and amplifying circuit, while the built-in ADC could still be used to acquire the RPS signal.(4) Draw the PCB circuit diagram of external AD7707chip, using Solidworks to print packaging model of portable system. Then we combine the data acquisition and analysis system and the optical detection system in this thesis, accomplishing the construction of the whole portable flow cytometry system and also have tested the system.