Endurance Exercise And Cyclosporine A On Rat Skeletal Muscle Fiber Composition Effects

Purpose: Endurance exercise and injection of cyclosporin A (CsA) interventioncalcineurin (CaN) signaling pathway, on rat skeletal muscle fiber type transformationfunction.Methods: Male SD rats for the study, adaptive training groups, SD rats were randomlydivided into four groups: control group (Group C) CsA group (S group), sports CsA group(ST group),simple exercise group (T group)10. Adaptive training of the four groups of SDrats for two weeks in the first1to2weeks to3to15weeks of endurance training, the S group,ST group at13-15weeks of the subcutaneous CsA15mg/kg/day (total3weeks). To stoptraining the day after SD rat soleus and extensor digitorum longus, SDS-PAGE andmeasurement of myosin heavy chain (MHC). Built in Nanjing, succinate dehydrogenase kittesting SDH activity and protein content with phosphorus method to measure the activity ofCaN, and activated T cell nuclear factor (NFATc1) measured by Western blotting.Results:SDH: ST and C groups compared to very significantly increased (P <0.05); the ST groupcompared with group C have a very significantly higher (P <0.01).Myosin concentration: the concentration of myosin of the rat soleus (SOL), compared tothe S and C groups decreased significantly (P <0.05); the ST group compared with group Cdecreased significantly (P <0.05). Rat long toe extensor (EDL) no significant difference in theconcentration of myosin.CaN: CaN activity of the rat soleus (SOL) in group S and group C compared withsignificantly decreased (P <0.05); the ST group and C group compared with the ST group isvery significantly decreased (P <0.01); compared with T group was significantly decreased (P<0.05). Compared to the rat extensor digitorum longus (EDL) of CaN activity, T and Cgroups have very significantly increased (P <0.01); compared to ST group and S group weresignificantly decreased (P <0.05); ST group and T group compared to very significantlydecreased (P <0.01). MHC: rat soleus muscle of MHC I, the ST group and C group compared with thesignificant rise of MHC II a significantly decreased (P <0.05). Rat extensor digitorum longus,the x of MHC II, in group S and group C compared to a very significant increase in MHC Ⅱd significantly decreased (P <0.01). Rat extensor digitorum longus of MHC Ⅱ x ST groupand S group compared with the very significant decreased MHC Ⅱ d is significantly higher(P <0.01).NFATC1: rat soleus NFATC1, in group T and group C compared with significantlydecreased (P <0.05); ST group and C group significantly decreased (P <0.01); compared tothe ST group and S group have a very significant decline (P <0.01).Conclusion:1. Injection of CsA can lead to reduced rat diet, weight loss, accompanied by thesubcutaneous tissue cysts and ulcers.2. Endurance exercise enhances aerobic oxidase activity of rat skeletal muscle SDH to befurther developed. CsA intervention did not affect the activity of SDH.3. Endurance exercise and injection of CsA can cause degradation of rat slow-twitchsoleus muscle myosin.4. Injection of CsA can inhibit the activity of CaN, endurance exercise can be caused bythe fast-twitch extensor digitorum longus CaN activity to increase, but no effect on CaNactivity of slow-twitch soleus muscle, the degree of intervention for different types of muscleinjection of CsA, CaN.5Increase in endurance exercise and injection of CsA could slow twitch soleus muscleof MHC I, MHC Ⅱ a, reduce, decrease in the fast-twitch extensor digitorum longus of MHCⅡ x, MHC Ⅱ d increases.6. Endurance sports slow-twitch soleus muscle Ca2+concentration increased, the cause ofCaN activity to improve the lot of NFATC1go phosphide into the nucleus. Injection of CsAinhibition CaN expression NFATC1to not enter the nucleus. Endurance exercise and CsAtogether soleus the cytoplasm NFATC1into a large number of the nucleus.