| ObjectiveIn this study, by observing the effect of IL-4espression in cartilage and the effect of improvement in articular cartilage ingury led by needle knife interventing the mechanics change of knee osteoarthritis (KOA) rabbit model patellar ligament. In order to explore a signal transduction mechanism of needle knife interventing KOA rabbit model to promote cartilage damage to repair based on IL-4. Provide evidence and guidance for the clinical application of needle knife therapy on knee osteoarthritis (KOA).Methods40normal New Zealand rabbits (6months old,male and female, weight2.25+-0.1kg) were randomly divided into four groups, named control group, model group, EA group, needle-knife group. Adopt fixing the left hindlimb in extension position for knee osteoarthritis (KOA) rabbit model. Six weeks later, Took the specimens10measure patellar ligament tension, stress relaxation and creep mechanical properties, analyze the mechanical changes of KOA rabbit patellar tendon before and after the intervention of the needle knife therapy. Test the content of IL-4in knee cartilage by ELISA, analyze the changes of IL-4content in cartilage before and after needle knife method improving the mechanical characteristics of the patellar tendon. Observe the morphology of each group cartilage, analyze the degree of improvement in cartilage damage after the needle knife improving patellar tendon mechanical properties.Result1Relaxation and creep experimental results of the patellar tendon:Seen from the analysis of the stress relaxation rate and creep rate, there is a significant difference between the four groups, the stess relaxtion rate of the needle knife group is smaller than the model group with a significant difference (P<0.05); the stress relaxation rate of the model group is greater than the normal control group with a significant difference (P<0.()5); there is a significant difference between the needle knife group and the normal control group (P<0.05); compared with the model group there is no significant difference in EA group; the creep rate of the needle knife group is greater than the model group with a significant difference (P<0.05); the creep rate of the model group is lesser than the normal control group with a significant difference (P<0.05); there is a significant difference between the needle knife group and the blank group (P<0.05); there is a significant difference between the EA group and the model group (P<0.05); As can be seen from the results, the patellar tendon stress relaxation and creep characteristics both change through2hours creep and stress relaxation tests in the KOA model, the needle knife group places between the blank group and the model group, suggesting that the needle knife method can effectively restore the stress relaxation and creep viscoelasticity. In the tensile mechanical test, the maximum stress, the maximum displacement, and elastic modulus all have statistically significance between the four groups(P<0.05; P<0.01). while the maximum strain has no statistical difference (P>0.05). Pairwise comparison, the maximum stress has significant difference between the needle knife group and the model group (P<0.05); there is pole significant difference between the model group and the blank group (P<O.01); the maximum displacement of the needle knife group has a significant difference compared with the model group (P<0.05); compared with the blank group there is a significant difference (P<0.05); there is a pole significant difference between the blank and model group (P<0.05); the differences of the elastic modulus between the needle knife and the model group is highly significant, the same as the EA group compared with the model group; there is a significant difference between the needle knife group and the’blank group (P<0.05), there is a pole significant difference between the model group and the blank group (P<0.01); From the results we can see that, the tensile mechanical characteristics of the patellar ligament in the model group changes significantly, the needle knife method can effectively improve this kind tensile mechanical characteristics.2ELISA for detection of the cartilage of the KOA rabbit in interleukin-4levels results show that:the content of IL-4in the model group cartilage is lower than the normal control group with a significant difference (P<0.01); he content of IL-4in the needle knife group cartilage is higher than the model group (P<0.05). the content of IL-4in the needle knife group cartilage is lower than the normal group (P<0.05); the content of IL-4in the EA group cartilage is higher than the model group, with a significant difference (P<O.05); the content of IL-4in the needle knife cartilage is higher than the EA group with a significant difference (P<0.05); As can be seen from the results, the content of IL-4in the needle knife cartilage gradually increased after the needle knife improving the ligament mechanical changes, but did not reached the normal group, which implys us that IL-4plays an important role in the cartilage repair.3The results of articular cartilage observing by light microscopyThe knee joint cartilage thickness of blank group rabbit is normal, the surface of cartilage is smooth, Multi-level cells are arranged in neat rows and in normal form. The stromal has no degeneration, the tide line is clear, there is no generation for pannus and there is no abnormal change under bone tissue.Degeneration and necrosis occurs in the model group cartilage cell, the surface of cartilage is not smooth, the individual remaining cartilage cells can be only seen, the cells are arranged irregularly significantly, there are some blood vessels through part of cartilage and pannus are generated, the level is disorder, chondrocyte ranks in nest, exfoliation and defect has dept to the tide line, the tide line is blurred or double tide line can be seen.cartilage surface is smooth, multi-level cells arranged in neat rows and morphology were normal, matrix degeneration, clear tide line, pannus formation, and bone underorganizations withont exception.Model group cartilage cell degeneration and necrosis, the surface is not smooth, only to see the individual the residual cartilage cells, cell arrangement was rrregular, level disorder, exfoliative defect depth of the tide line, the chondrocyte nest, the tide line is blurred or double tide line, part of the cartilage blood vessels through and pannus generated. Needle knife group cartilage cells arranged in neat rows, the tide line is clear, smooth cartilage surface, and no pannus formation; The EA group chondrocytes sections in accordance with the Mankin method score, four sets of ratings overall differences were significantly different (P<O.01). The pairwise comparison needle knife group the degree of cartilage damage better than the model group (P<0.01). there are differenes compared with the control group. The needle knife law cartilage play a protective role, but did not reach normal leves.ConclusionThrough loosing adhesions of patellar tendon by needle knife, the biomechanical properties of ligaments has been adjusted effectively, and the mechanical balance of the knee joint are restored. At the same time, peripheral improvement of mechanical equilibrium has an impact on the IL-4expression in cartilage, whiletime plays a role on the repair and improvement to the articular cartilage damage. Therefore, we have reason to believe that, after the needle knife improving the mechanical changes of ligament, It plays the role of cartilage protective treatment by mechanical signal transduction, however chondrocytes mechanical signal transduction pathway is one of the crucial one based on IL-4, this may be one of the needle knife treatment of KOA mechanical signaling transmission mechanisms. |
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