Maxingshi Sweet Soup Of The Two Methods Of Pan-fried Income One Antibacterial And Effect On Type A Influenza Virus Infection Mice Of Experimental Research

Objective:A Comparative Study of Two boiling method (ephedra first boiling and four herbs with boiling) the proceeds Maxingshigan decoction of the antibacterial and influenza A virus infection of mice.Methods:Antimicrobial experiments:The agar diffusion test and tube method were respectively used for examination of the antibacterial effects of the decoction which ephedra decocted first (D-ephedra) and the decoction which those four kinds of herbs decocted at the same time (D-4) on S. aureus, S. epidermidis, E. coli, a-hemolytic streptococcus,(3-hemolytic streptococcus.The antibacterial effects include the inhibition zone diameters, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Anti-A-type influenza virus experiments:Nasal inoculation method to establish the type A influenza virus lung infection in mice model, Experimental set normal comparison group, Model comparison group, ephedra first boiling group, four herbs with boiling group, Oseltamivir group,7days of continuous given medicines by intragastric. Respectively,4days and7days after infection to detect the body weigh of mice, on the7th day, routine testing Lung weight index,Thymus index, Spleen index; detected TNF-a,IL-6concentration level by ELISA,observation of lung changes by optical microscopy techniques, lung tissues were observed ultrastructure by transmission electron microscopy techniq ues.Results:Antimicrobial experiments:The diameters of inhibition zones formed in D-ephedra against S. aureus, S. epidermidis and (3-hemolytic streptococcus were remarkably larger than D-4(P<0.01); The MIC of D-ephedra effect on S. epidermidis and the MBC of D-e effect on S. aureus and S. epidermidis were lower than the MIC and MBC of D-4. Anti-A-type influenza virus experiments:①Body weight:4days and7days after infection,compared with the normalcomparison group, model comparison group significant weight loss(P<0.05),7days after therapy, compared with the model comparison group,Oseltamivir group, ephedra first boilinggroup significant weight gain(P<0.01or P<0.05).②Lung index:ompared with the normal comparison group,the mouse lung index of model comparison group increased significantly (P<0.01).compared with the model comparison group,Lung index was significantly reduced in all treatment groups (P<0.01or P<0.05),The significant difference (P<0.01) about of the ephedra first boiling group compared with the model comparison group is higher than thefour herbs with boiling group compared with the model comparison groupon the Significance(P<0.05).㏕hymus index:compared with the normal comparison group, model comparison group significant loss on the thymus index of mice (P<0.01);compared with the model comparison group, Thymus index was significantly higher in the treatment groups (P<0.05);Among the treatment groups, do not find statistically significant difference(P>0.05)。④Spleen index:compared with the normal comparison group,the mouse Spleen index of model comparison group increased significantly (P<0.01); compared with the model comparison group, Oseltamivir group spleen index was significantly higher (P<0.05), ephedra first boiling group and four herbs with boiling group do not find statistically significant difference (P>0.05)。⑤ytokines:compared with the normal comparison group, The levels of serum TNF-α, IL-6increased about of model comparison group (P<0.01). compared with the model comparison group, The levels of serum TNF-α, IL-6is significantly decreased among the treatment groups (P<0.01, P<0.05), compared with ephedra first boiling group and four herbs with boiling group,The levels of serum TNF-a is lower and significant difference (P<0.01); The levels of serum IL-6are not significantly difference(P>0.05).⑥Lungpathological changes:compared with the normal comparison group,the mouse lungs normal tissue structure disappeared,the alveolar inflammatory cell infiltration. Compared with the model comparison group,Lung tissue of all treatment groups pathological change significantly improvely,Among Oseltamivir group and ephedra first boiling group,the level of Lung disease in mice is lighter than four herbs with boiling group.⑦Lung tissue ultrastructure:compared with thenormal comparison group, the mouse lung tissue ultrastructure of model comparison group was damaged,theperformance of the nuclear membrane and the plasma membrane are blurred,reduced organelle, the mitochondrial cristaeis reduce ordisappear,Endoplasmic reticulum emerges vacuolar lesions,there are a large number of virus particles in the cytoplasm, each treatment group lung tissue structure has been improved.Among Oseltamivir group and ephedra first boiling group,the extent of Lung tissue ultrastructure diseasein mice is completer than four herbs with boiling group.Conclusion:the ephedra first boiling decoction is more effective than the four herbs with boilingdecoction on againning type A influenza virus.