Antai Oral Liquid On A Tumor-burdened Survival Quality And Urgent Toxicity Experiment In Mice

Purpose: Cancer is one of the major diseases of harm to human health, theincidence rate showed an increasing trend. Occult malignant tumors, earlydifficult to find cancer patients, approximately70%－80%in late, most patientshave lost the chance of early radical surgery. Western three treatments formalignant tumors have a better short-term effect, but its side effects, seriousharm to the patient’s righteousness. By retrieving the database, according tothe commonly used prescription of years of clinical experience, combined withthe "Chinese heritage auxiliary systems, drawn Aetna oral prescription. To seekAetna oral liquid on the survival time of mice, the inhibition rate, as wellas its acute toxicity reaction, this experiment gives the S180mice Aetna oralsolution the Zhenqifuzheng particles, cyclophosphamide, in order to observe theanimals in each group the general state of survival, the inhibition rate, andimmune organ spleen, thymus quality and security.Materials and methods:1.Material: Kunming mice, half male and half female, weighing18－22g, S180tumorcells, Aetna oral solution (Poria the psoralen Yiyiren Astragalus AtractylodesPinellia dried tangerine peel, etc.), cyclophosphamide, Zhenqifuzheng particles,saline, electronicscale.2.Methods:2.1model: vaccination7days ascites tumor growth in mice suction milky thickascites, diluted with saline ratio of1:3, the plate count of tumor cells andblood cell counts, adjust the number of tumor cells1×107/ml., made of a tumorcell suspension. Before the inoculation of tumor cells with0.2%trypanblue/trypan blue staining to count the live tumor cells＞95%. Subcutaneous 0.2mlS180cell suspension (approximately2×106cells) in the mouse right frontarmpit, made of S180tumor model. Inoculated with a total of40.2.2Aetna oral liquid on the survival time of tumor-bearing mice experiment:Animals were randomly divided into four groups (n=10), male and female.: AndAetna oral solution group, Zhenqifuzheng particle group, cyclophosphamide group,model control group. Aetna oral solution group tumor-bearing mice were given0.4ml/(only ig Day) Zhenqifuzheng particle group of tumor-bearing mice given0.4ml/(only the day) administered orally, cyclophosphamide group charge tumorsin mice by intraperitoneal injection of cyclophosphamide (20mg/kg day), modelcontrol group: normal saline0.4ml/(only day). Groups after tumor inoculationthe first two days of dosing, once daily. Damage in order to ensure administrationof the amount is not in the stomach of mice administered orally before the fastingand water deprivation2hours. The daily said the body weight of mice to observethe coat color, activity, food intake, until the death of the previous day tocalculate gain ratio. Counting the day of tumor inoculation to model the controlmice all died the end of the experiment, record the time of death, the calculationof the prolonged survival rate. Prolonged survival rate=(treatment group meansurvival days-the model control group, the average survival days)/Model controlgroup the average survival days×100%2.3Aetna oral liquid on the tumor-bearing mice tumor growth inhibitionexperiments: packet administration with the experimental one. Weigh all the bodyweight of mice24hours after the experiment12days administration of the endof, and then executed, quickly stripping the tumor, said whichever weightinhibitory rate was calculated. The inhibition rate=(model control group, tumorweight-tumor weight of treatment group)/tumor weight of the model controlgroup×100%. The anatomy remove the spleen, thymus, and to get rid of the fattissue, the use of electronic scales called weight, and calculate the spleenindex and thymus index. Spleen index=spleen weight/body weight of mice (mg/g), the thymus index=thymus weight/body weight of mice (mg/g).2.4Aetna oral solution acute toxicity test on the tumor-bearing mice: healthymice were40, were randomly divided into two groups, half male and half female.Aetna oral gavage group: Aetna oral solution for the mice fed the maximumconcentration (4.9g/ml), gavage0.4ml/10g weight at once, three times in a row,an interval of4hours. Gavage fasting can not help but water12hours beforethe interval during feeding. Control group: gavage the same amount of drinkingwater, remaining the same treatment group., Feeding one week afteradministration, observed in mice hair, activity, diet, and second, and death.Anatomical observation of the change of heart, liver, spleen, lung, kidney andother organs of the organization in eight days of the experiment, mice weresacrificed.Results:1.An oral solution Aetna survival of tumor-bearing mice experiments, comparedwith the model group survival of tumor-bearing mice by Aetna oral solution, oralsolution Aetna, Zhenqifuzheng particles can significantly prolong the survivaltime of tumor-bearing mice, prolonged survival rates were51.67%,32.78%. Thereare significant differences (P <0.05). The Aetna oral solution to extend thelifetime of the better than Zhenqifuzheng particles.2.Aetna oral solution tumor-bearing mice tumor growth inhibition experimentsAetna oral solution can inhibit tumor growth in tumor-bearing mice, the weightof the thymus and spleen of tumor-bearing mice with the model group comparedwith Aetna oral liquid Zhenqifuzheng particles, and cyclophosphamide are theinhibition of tumor growth inhibition rates were38.3%,32.5%,81.7%. There aresignificant differences (P <0.05).Aetna oral solution Zhenqifuzheng particlescan increase the quality of the thymus and spleen of tumor-bearing mice thymusindex and spleen index were1.71±0.45,1.43±0.48and5.58±0.68,5.23± 0.96, and enhance the tumor-bearing mice Sofitel heterosexual immune function.3. Aetna oral liquid on acute toxicity response in tumor-bearing mice experiment，Aetna oral liquid on acute toxicity experiments, the acute toxicity of the Aetnaoral solution is very small, can not be measured by intragastric administrationto mice the LD50, the activity of the mice, hair, water, stool, etc. with thecontrol group was no exception.Conclusion:1. Aetna oral liquid phase can significantly prolong the survival oftumor-bearing mice.2. Aetna oral solution can inhibit the tumor-bearing mice, tumor growth, andimprove immune function through the increase in thymus weight.3. Aetna oral solution is less toxic, by intragastric administration can notbe measured median lethal dose (LD50) in mice, demonstrate safety.