Security Spread Traditional Chinese Medicine Compound Experiment Research For The Treatment Of Chronic Pharyngitis

Purpose: use density is2.5%ammonia in SD rats directly spraymethod pharyngealmucosa manufacturing chronic pharyngitis in the rat model, and the appraisalmodel of the animals. Main observed the different doses of security for chronicpharyngitis scattered TCM compound in the rat model of the relevant basic index,pathological form blood and blood rheology influence. Observe different dosesof TCM compound security scattered chronic pharyngitis pharyngeal diseasedtissue in the rat model of epidermal growth factor (EGF) and1-β interleukin(IL-beta) the influence of the content.Materials and methods: the2.5%of ammonia in60rats directly spray methodpharyngeal mucosa manufacturing rat model of chronic pharyngitis, random willbuild model animals into model group, the placebo group, the security of thelow-dose scattered TCM compound, scattered in the security herbal dose groupof security, scattered TCM compound high dose group of group and pharyngitis,another set of normal. Observation rat models of different groups of routineblood, blood rheology index, light-microscopy rats pathological morphology ofthe pharyngeal mucosa on change and immunohistochemical determination methodof rats in the pharyngeal lesions mucosa epidermal growth factor (EGF) and1-βinterleukin (IL-beta) content.Results:1. The third day big rat made mode began to appear clinical symptoms,15dayssymptoms. The forehead, seen in the rat model chronic pharyngitis pharyngealmucosal is chronic inflammation change. Blood tests show animals adopted aroundin chronic inflammatory cells (lymphocytes, mononuclear cells) number ofsignificantly increased. The security of traditional Chinese medicine and pharyngitis scattered treatment two weeks after, rats clinical symptoms improved,pharynx ministry mucosal hyperemia improved obviously, in the peripheral bloodchronic inflammatory cells (lymphocytes, mononuclear cells) number ofsignificantly reduced, especially in scattered TCM compound high security dosegroup of change significantly.2. Blood rheology model test hint of the rats made blood viscosity, have certainof change, get together. The security of traditional Chinese medicine andpharyngitis scattered treatment two weeks after rat peripheral blood viscosity,gather and solidifying, pathological changes of the ease, especially inscattered TCM compound high security dose group of change significantly.3. By light microscopy see: build module rats a pharyngeal mucosa chroniccongestion, epithelial cells edema, mild add thick, it shows diffuseinflammatory cells infiltrating, blood vessels around more lymphocytes andmononuclear cells infiltration, also visible white blood cells and plasma cellsinfiltration. The mucous membrane obviously connective tissue hyperplasia. Havea wide range of connective tissue and lymphoid tissue hyperplasia, submucosalgland has not seen the obvious pathological changes, see the scattered in theconnective tissue more inflammatory cells infiltrating, skeletal muscle has notseen the lesions. The security of traditional Chinese medicine and pharyngitisscattered treatment two weeks after, the pathological change better, especiallyin scattered TCM compound high security dose group of change significantly.4. Rats pharyngeal mucosal immunohistochemical group showed that the model ofepidermal growth factor was obviously reduce, interleukin1-content increasedsignificantly. And the epidermal growth factor, interleukin1-content and theblank group quite.Conclusion:1. The SD rats chronic pharyngitis model is successful. 2. Security guards scattered TCM compound can correct change pharyngeal chronicinflammation. Security guards scattered TCM compound can reduce the animalsadopted around in chronic inflammatory cells (lymphocytes, mononuclear cells)of the quantity, reduce blood vessels permeability and pharyngeal inflammatorycells oozy, can improve the blood rheology, enhance the immune function. Caneffectively inhibit inflammatory mediators interleukin1-release, and increasesthe tissue damage after repair factor of epidermal growth factor is generated.Show that security guards scattered herbal treatment of chronic pharyngitis roleits mechanism may be through the antibacterial, anti-inflammatory, improvepermeability, reduce blood vessels pharyngeal inflammatory oozy, improve bloodrheology, enhance the immune function, suppress the occurrence of pathologicalchanges pharyngeal inflammation, improve blood coagulation, get together thesticky, state, increase the content of epidermal growth factor and preventinterleukin1-release, link to work to fulfill.