| Objective:The effects of Xuefuzhuyu decoction, Svate-3and NGF on the hippocampal neurotransmitter changes in the brain of the MCI rat have been studied in order to provide a theoretical basis for clinical treatment of MCI patients.Methods:150male, healthy, Wistar rats, weighing250-300g, were fed composite particles and drinking water. After five day of environmental adaptation, the rats were randomly divided into four groups:(1)10rats for normal control group;(2)20for the blank control group;(3)20for MCI model group;(4)100for treatment group. And depending on the drug treatment, the treatment group was again divided into group A、B、C、D、E and20rats for each group which were treated with different drugs. Group A:treated with Xuefuzhuyu decoction;Group B:Xuefuzhuyu decoction+Svate-3;Group C:Xuefuzhuyu decoction+NGF;Group D:Svate-3+NGF;Group E:Xuefuzhuyu decoction+Svate-3+NGF. In the research, the model of rat MCI was created by using the method of microembolus-blocking method. After operation, normal saline of1ml/100g. d was given to each of the rats in control group and model group by means of gavage, and Xuefuzhuyu decoction of1ml/100g. d was given once a day to each rat of the5treatment of the proportion group. The dose of normal saline and Xuefuzhuyu decoction givento the rats was equivalent to adult human body50Kg BW each. NGF (NGF1000BU soluble in2ml saline) was used by means of once daily intramuscular injection;Svate-30.025u/200g was used by means of once daily tail vein iniection. Each of the treatment group was drawn at the time of Id and7d. Rats were weighed before and after the experiment in order to observe changes of rats weight and neurological behaviors. Immunity and enzyme group optical mirror were used to observe the quantity of hippocampal CA(?) neurons form, the number of NOS positive reactants and the NHB positive reactants.Results1. Weight and neurobehavioral observation results:compared with those before the experiment, the weights of the rats’in blank control group, mode] group and treatment group decreased. Neurological behaviors of the rats in normal group and blank controlgroup changed, varying degrees of neurological dysfunction appeared in the model group and treatment group.2. Light microscopy results:(1) Postoperative Id:compared with those in normal control group, the number of the survived neurons decreased significantly (P<0.01) in model group and treatment group; there was no significant difference (P>0.05) in the blank control group. compared with that of the model group, nNOS positive neurons of each of the treatment group decreased significantly (P<0.01). Compared with that of mode] group, the number of NKB positive neurons of each treatment had an obvious increase (P<0.01).(2) postoperative7d:compared with that of model group, surviving neurons of treatment group increased significantly (P<0.01). nNOS-positive neuron number of treatment group-C、D、E, was close to that of the normal control group(P>0.05). compared with that of the model group, the number of NKB-positive neurons of each treatment group increased (P <0.01).3. Electron microscopy results:on slices of hippocampal CA, region normal group, neurons were stratified arranged; matrix was uniformly: nucleus was large and round; nuclear membrane was integrated; nucleolus was clear;organelles in the cytoplasm was rich. On slices of hippocampal CA, in the model group, the neurons was disordered, nucleus narrowed; nuclear membrane was partly dissolved; mitochondria swelled:endoplasmic reticulum expanded. slit-like or dense striped. ConclusionNOS and NKB were both involved in brain damage. The drugs in each treatment group could accelerate the recovery of damaged neurons, and the effect of treatment group-E was obvious. |
PDF Full Text Request