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Low Salt Diet Relevance To Wound Healing In Rats Experiment Research

Posted on:2013-08-18Degree:MasterType:Thesis
Country:ChinaCandidate:Y ZhangFull Text:PDF
GTID:2244330395979011Subject:Traditional surgery
Abstract/Summary:PDF Full Text Request
Purpose:Chinese medicine lotion promote wound healing in rats infected withthe purpose of this experimental study on the basis of the theory of Chinesemedicine, combined with modern research results through animal experiments,observe the effect of lotion for rats before and after treatment back woundgranulation tissue in the wound inflammatory changes in the efficacy ofChinese medicine lotion mechanism.Materials and Methods:Experimental rats materials: clean-60,8-10weeks of age, body weight200-240g。Feed: normal diet.Chinese medicine lotion drug components: mirabilite25g25g borax Chuanjiao alum10g25g10g purslane.Main instruments and reagents experimental lysozyme Kit (LZM) test box (withst-andards)(turbidity tube) acid phosphatase kit biological en-gineering therat tumor necrosis factor (TNF-α) enzyme immunoas-say kit Belgium wound pusin the wound of PH value and the content of lysozyme and wound granulation tissuein acid phosphatase (ACP) and tumor necrosis factor (TNF-α) content.Experimental Methods:1animal packet delivery method and60adaptive animal feeding experimentafter one week, were rando-mly divided into2groups. Control group: LEICeftiofur Nour solution wound clean, sterile gauze dressings, dressing one day.Experimental group: Chinese medicine and topical lotion fixed, sterile gauzedressings, dressing one day.2model of wound healing in rats two days before the establishment of backsurgery in mouse skin site, for when ether anesthesia, surgical site using iodinedisinfection, the Rat back to the center position on the side of a diameter of2cm incision around the fusiform excision full-thickness skin and subcutaneoussuperficial fascia muscle until bleeding in the wound adding1ml of fecal (fresh stool10g,5ml normal saline, blending, centrifuged and supernatants from,the existing service is used) on cotton covering the w-ound, the gauze coveringthe fixed.48h after the removal of cover, the wound is Nongshui see, fecalste-nch, the modeling success. Perturbation two rabbits were free of water,feeding single cage.Results:1.13,5,7,12after the first one, wound pus in the wound of PH value andthe content of lysozyme Turbidi-metric method from standard application.2.after the first day of7,9,12wound granulation tissue (ACP) and (TNF-α)content.Conclusions:The above indicators to change the description fo the low-salt diet groupto reduce wound inflammation,accelerate wound healing.
Keywords/Search Tags:ACP, TNF-α, PMN, LY, Fn
PDF Full Text Request
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