Carbon Nanotube Modified Electrodes Study The Effect Of Aluminum Form Compound Of Several Kinds Of Enzyme Activity

Aluminum exists mainly in the form of silicate and oxide in the mineral, all sorts of environmental problems such as acid rain during recent years force lakes, rivers, soil sufferring different degree of change, thus the aluminum becomes dissolved out from rock and soil by various bioactive form presenting in the environment. Many in vivo studies have demonstrated that aluminum can inhibit all kinds of enzymes activity which included in the Krebs cycle and glycolysis, which seriously hinder the normal operation of mitochondrial cell energy supply systems getting into the human body of aluminum through many ways will cause a series of neurological disease, such as anemia, osteomalacia disease, alzheimer’s disease, Parkinson’s disease. Therefore, it is very important to study human’s daily life containing the element. In recent years, people have found nanoparticles can cause potential threats on natural environment and human health, so the safety of nanoparticles has received more attention and research. This paper using carbon nanotubes modified glass carbon electrodes to research the effects of nano and other forms of aluminum compounds on Glutathione Reductase (GR) activity which depends on coenzyme Ⅱ (NADP+/NADPH), and use the Circular Dichroism method to study the mechanism, adopting reduced graphite modified electrodes to explore the effcts of aluminum compounds on aldehye dehydrogenase (ALDH) activity depends on coenzyme I (NAD+/NADH). Separately, using the both above electrodes to explore the effects of aluminum form compounds on alcohol dehydrogenase (ADH) activity, using chemical calculation method for the further discussion. The experimental results are described as followed:1. With carbon nanotubes (MWNTs)/chitosan CHIT modified glassy carbon electrode and reduced graphite oxide (rGO)/CHIT modified electrodes, both of these two electrodes can obviously reduced the over potential of NADPH and NADH on the bare glassy carbon electrode, use timing current i-t method, by detecting catalytic current changes of NADPH and NADH, examing the influence of aluminum compounds on GR, ALDH and ADH activity, separately. The experimental results showed aluminum species have certain inhibition on GR, ALDH and ADH activity.2. Different forms of Aluminum have different impact on enzyme activity, but also can be influenced by solution pH value. When the pH value of the buffer is below7, Al(Ⅲ) presents obvious inhibition on GR, ALDH and ADH activity, simultaneously as the system pH value increases, reduced inhibitory effect deceases. That is because when the solution is in low pH, the main form of Al (Ⅲ) can interact with GR system. So conversely, buffer in a neutral pH about7, Al13exist most obvious inhibition to GR, ALDH ADH activity. This can be attributed to Al13is most stable under this acidity, and under the partial acid and alkali conditions, the weaken effcts of polymerization degree of Al13makes it more unstable.3. Choose Circular Dichroism to study the mechanism of Al (Ⅲ) and Al13on GR activity, the results showed that Al (Ⅲ) and Al133can change conformation of GR. In addition, there are fluorescence spectrum and quantum chemical calculation methods studying the effcts of Al (Ⅲ) and Al13on ADH enzyme activity, the experimental results show that Al (Ⅲ) and Al13can cause conformation transformation of both ADH and NAD+.