| Objective: This experiment is to study the effect of curcumin on behavior of autismmouse and the expression of BDNF.Methods:(1) Reference to Tomasz Schneider’s method，the model group of femalesreceived a single intraperitoneal injection of sodium valproate at the pregnant day of12.5;Control group was injected with the same amount of physiological saline at the same time.The offspring of the model group females was maked as the sodium valproate group (VPAgroup); The offspring of control group females was maked as the saline group (NS group).The born day wes designated as the first day of postnate (P1). The offspring of the twogroups was tested in developmental, behavioral, and morphological.(2) Used theimmunohistochemical methods and image analysis techniques to observe the expression ofBDNF in autism model mouse cerebral cortex on P1, P7, and P14, p21, P35and P49.(3)Randomly selected animal model of autism (n=40)and offspring of the NS group(n=10) atage of35days, the saline group rats wes designated as normal control group (Con), Theanimal model of autism were randomly divided into four groups: vehicle-control group(VC), low dose of curcumin (10mg/kg，CUR I), intermediate dose of curcumin (30mg/kg，CUR II) and high dose of curcumin (50mg/kg，CUR III). The curcumin groupreceived intraperitoneal injection of curcumin for two consecutive weeks, the solvent controlgroup and normal control group was given PBS with volume of0.1%DMSO. Observed theimpact of different doses of curcumin on the autism mouse behavior, and usedimmunohistochemical staining to observe the effect of curcumin on the expression of BDNFin cerebral cortex.Results:(1) This study found that the mouse of the VPA group showed the followingexception: Aspect of developmental,compared with NS group,VPA group rats presencedlow birth weight, eye opening delays, poor coordination response, and abnormal directiontendency. In the developing brain, the brain of VPA group mouse was overgrowth in earlytime, but increase with age, its development declined; Aspect of behavior, VPA group ofmice showed number of Social behavior decreased, lantency of social behavior extended, duration of social behavior shorten,repetitive activities increased and low learning andmemory; Aspect of morphological, Purkinje cells reduced.(2) This study showed thatBDNF expression of VPA group was significantly higher than NS group with thecorresponding parts of temporal cortex at postnatal day1d,7d and14d (P<0.05); andcompared with the NS group, BDNF expression of VPA group was significantly lower atpostnatal day35d and49d (P<0.05).(3) The effects of CUR I on the behavior of autismmouse and BDNF expression of autism mouse in brain temporal cortex is not significant (P>0.05); CUR IIcan partially improve the behavior of autism, it can reduce the latency of socialbehavior (P<0.05), reduce the cumulative time of the grooming behavior (P<0.05),but it hadno significant effect on BDNF expression (P<0.05); the role of CUR III to improve thebehavior of autism mouse is the most significant,it can increase the number of socialbehavior (P<0.05), decrease the latency of social behavior (P<0.05) reduce the cumulativetime of the grooming behavior (P<0.05), improve learning and memory (P<0.05),increaseBDNF expression of autism mouse in temporal cortex (P<0.05).Conclusion:(1)These results suggest that BDNF participates in the pathogenesis ofautism, and may be related to the incidence of autism.(2)Curcumin have a therapeutic effecton mouse with autism,it can be improved abnormal behavior of autism mouse to someextent, the mechanism may be to play a role by increasing the expression of BDNF.(3)Thetherapeutic effect of curcumin on autism have a positive correlation with dose of curcumin... |
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