Preliminary Exploration Foxpl And Ctgf In The Role Of Sugar Metabolic Regulation

Maintaining the blood glucose within a relatively narrow range has evolved for a long time. This involves the production of glucose by the liver, from glycogenolysis and gluconeogenesis, and the peripheral clearance of glucose by tissues such as the skeletal muscle, adipose tissue, and the splanchnic bed, including the liver. The liver plays a important role as a producer/consumer of glucose. The peroxisome proliferative activated receptor-g co-activator1α (PGC1α), a transcriptional co-activator whose function is required for the robust activation of some of gluconeogenic genes expression in hepatic cells. Hepatic gluconeogenesis is inappropriately activated in diabetes mellitus. Foxp1,a member of Fox family,its full name is forehead box p1and it is consisting of a winged-helix DNA binding domain and a homologous DNA binding-dependent N-terminal transcriptional repression domain.The gene has a broad range of functions and plays an important role in cardiac and lung development, B-cell development and macrophage differentiation. FOXP1is implicated in malignancy.Analyzing the chips,we found the expression of Foxpl is different under different nutritional conditions leading a speculation of Foxpl involved in the metabolism of glucose. We have proved Foxpl regulated the expression of PGC1α、PEPCK、G6PC and GCK.Overexpression of Foxp1in the primary hepatocyte could improve the sensitive of insulin.Finally, we conclude that Foxp1plays a important role in the blood glucose control and may be a therapeutic targets of diabetes mellitus. The protein connective tissue growth factor (CTGF), also known as CCN2, is a heparin binding36-to38-kDa cysteine-rich protein and a member of the highly conserved CCN early response gene family of peptides. To construct and identify a adenovirus vector of the expression of Ctgf and to study the function of Ctgf related to the metabolism of glucose and lipid. Cloning the over-expression plasmid of Ctgf and the Ctgf sequences were cloned into pAdTrack-CMW vector. The reformed E.coli BJ5183sensitive bacterials which contain pAdEasy-1were transformed with lined vector cutted by Pme I enzyme. The recombinant adenovirus vector was cutted with Pac I enzyme and obtained, then transfected293A cells to produce virus. Through three times amplification, the adenovirus infected the primary hepatocytes to see the efficacy of the adenovirus and detect the expression of Ctgf. Through starving the mouse during several different time slices, the RNA of the hepatic of these mouse were isolated then did the real time PCR to detect the change of the expression of Ctgf under different nutritional conditions. Successfully producing the adenovirus of Ctgf and the efficacy of the infection of the adenovirus is above90%.The expression of the Ctgf is different in different nutritional conditions and there is coincidence between Ctgf and PGC1α.The peroxisome proliferative activated receptor-g co-activator1(PGC1α), a transcriptional co-activator whose function is required for the robust activation of some of gluconeogenic genes expression in hepatic cells. Hepatic gluconeogenesis is inappropriately activated in diabetes mellitus. Starving24h stimulates gene Ctgf3.38±0.51and48h3.95±0.57(p<0.05). So Ctgf may be related to the metabolism of glucose and lipid.