Scd163 And Nk Cell Activity In The Secondary Meaning Of White Blood Cells Sex Is Lymphatic Histiocytosis

ObjectiveThe hemoglobin-haptoglobin scavenger receptor(CD163), as a novel biomarker,is a monocyte/macrophage-restricted transmembrane protein of the cysteine-richscavenger receptor family. The expression of sCD163molecules in the plasmasignificantly increased due to infection, cancer, autoimmune diseases and otherunderlying causes which can induce excessive activation of macrophages. This studyis aimed to investigate the significance of sCD163in secondary HLH (sHLH)patients, including diagnosis, probing the undelying disease, guiding treatment andevaluating the therapeutic effects.MethodsSerum specimens of32patients with newly diagnosed sHLH were collected.Meanwhile, serum specimens of9sHLH patients before and after treatment were alsocollected.In addition, serum specimens of15healthy control were collected. ThesCD163level was detected by enzyme linked immunosorbent assay (ELISA).ResultsThe average sCD163levels of patients in sHLH group was4,885.7ng/ml(2,012.0-12,826.8ng/ml), which was significantly higher than the levels of healthycontrols848.5ng/ml(558.4-1,505.2ng/ml)(P＜0.05). Among the41cases of sHLH,the average sCD163levels of patients secondary to malignant tumors (n=12) was6,969.3ng/ml(2,281.0-12,826.8ng/ml), which was significantly higher sCD163levelsthan patients secondary to infections(n=23) and autoimmune diseases (n=3)(P＜0.05).Among the9cases of sHLH with specimens before and after treatment, we found thataverage sCD163levels of all the9cases after treatment were1,791.6ng/ml (768.5-4,375.2ng/ml), which were signicantly lower than the levels of beforetreatment7,358.7ng/ml(3,885.0-12,826.8ng/ml)(P＜0.05). Our data also showed thatsCD163level in sHLH patients had positive correlation with serum ferritin andsCD25levels, and there was no correlation between sCD163level and white bloodcells, hemoglobin, triglycerides, fibrinogen and NK cell activity.ConclusionIt is concluded that sCD163plays an important role in the diagnosis, probing theundelying disease, guiding treatment and evaluating the therapeutic effects amongsHLH patients. For suspected HLH patients, especially in the earlier stage of diseasewhich failed to meet HLH-2004diagnostic guidelines, sCD163detection may behelpful for earlier diagnosis. In addition, the sCD163level can predict the underlyingdisease of sHLH, and patients with MAHS showed higher sCD163levels thanpatients with IAHS and MAS. As to the clinical courses of sHLH, sCD163level canreflect the disease status accurately. Dynamic detection of serum sCD163level can behelpful for grasping the opportunity for treatment implementation, timely adjustingthe therapy regime and improving the prognosis of sHLH. ObjectiveNK cells play important roles in innate immunity against tumors and infectionsof the host. As degranulation occurs, secretory lysosomes are released, and thelysosome-associated membrane protein-1(LAMP-1, CD107a) is transported to thesurface of NK cell, thus making it possible to identify NK cells which have beenactivated for degranulation. The study focused on the comparative analysis of flowcytometry and LDH release assay, both can detect peripheral blood NK cell activity,and the significance of NK cell activity in the sHLH.MethodsBlood samples of22patients with newly diagnosed sHLH were collected,including7patients before and after treatment. In addition, blood samples of15healthy control were also collected. Flow cytometry and LDH release assay wereindividually utilized to detect peripheral blood NK cell activity.ResultsFlow cytometry following CD107a antibody labeling in patients with sHLHshowed that peripheral blood NK cell activity was11.7±4.85%, which wassignificantly lower than the normal control group level(31.72±5.93%). LDH releasemethod showed that peripheral blood NK cell activity was21.7±5.45%, which wasalso significantly lower than the normal control group level(43.17±5.39%).Peripheral blood NK cell activity of7patients after treatment was significantly higherthan before treatmen（tP＜0.05）. CD107a expression detected by flow cytometry andNK cell activity determined by LDH release method showed that there is significant correlation between two sets of data(r=0.75，p<0.05).ConclusionsThe results of comparison of flow cytometry following CD107a antibodylabeling and LDH release assay showed that flow cytometry need less number ofeffector cells, with better stability and repeatability, demonstrating advantages inclinical specimens testing. The early utilization of CD107a detected by flowcytometry can evaluate NK cell activity, which will contribute to early diagnosis.