Silence Glypican - 3 Genetic Enhancements Hepg2 Liver Cancer Cells To Chemotherapeutic Drugs Induced Apoptosis Effect Of Sensitivity

Glypican-3(GPC3) is a heparan sulfate proteoglycan that is bound to the cell membrane by a glycosylphosphatidylinositol(GPI) anchor,which is a member of Glypicans family. Studies showed that GPC3was overexpressed in hepatocellular carcinoma(HCC) cells and involved in the onset and growth of HCC.OBJECTIVE:Gene silencing of glypican-3was performed in HCC cells HepG2, investigate the sensitivity to5-FU and CDDP and the effect of apoptosis induced by5-FU.METHODS:We generated a hepatocellular carcinoma cell line stably transfected with plasmids encompassing shRNA targeting GPC3,and control was used.Western blot analysis was performed to assess the expression of GPC3.The sensitivity to5-FU in HepG2was evaluated by MTT assay. Flow cytometer was used to observe the effect of apoptosis induced by5-FU.RESULTS:The expression of GPC3was negative in HepG2cells transfected with shRNA. HCC cell line with silenced GPC3expression was more sensitive to5-FUand CDDP (p<0.05);and5-FU-treated shGPC3cells showed a significant increase in apoptosis(p<0.05).CONCLUSION:Increased GPC3expression in HCC cell line may play a protective role against apoptosis, and the down regulation of GPC3may be a potential target to increase sensitivity to5-FUandcDDP-induced apoptosis in hepatocellular carcinoma cells.