Nlrp3 Inflammation Complexes In The Training Of High Sugar Expression Of Renal Tubular Epithelial Cells And Its Relationship With Apoptosis

Objective To Study the expression of NLRP3inflammasome in high glucose culturedNRK-52E cells and its relationship with cell apoptosis.Method NRK-52E cells were cultured in high glucose and randomly divided into5groups:(1) control group: Culture medium containing5.6mmol/L D–glucose;(2) isotoniccontrol group: Isotonic medium containing5.6mmol/L D-glucose,24.4mmol/L mannitol;(3) high glucose group: medium containing30mmol/L D-glucose;(4) high glucose+negative control group: cells transfected with recombinant plasmid containing unrelatedsequences in cultured in high-glucose medium;(5) high glucose+interference group: cellstransfected with NLRP3for the expression plasmid in cultured in high-glucose medium. After48hours, NLRP3,ASC,Caspase-1,3protein expressions were detected by Western blotting,The apoptotic rate of NRK-52E cells was measured by flow cytometry.Results high glucose could increase the NRK-52E cells NLRP3inflammationexpression and caspase-3protein expression(P<0.05); cell apoptotic rate increased(P<0.05).Through the specific inhibition of SiRNA NLRP3protein expression, the levels of cellularNLRP3inflammation and Caspase-3expression decreased in high glucose+interferencegroup(P<0.05);cell apoptotic rate decreased obviously(P<0.05).Conclusion high glucose could increase NRK-52E cells NLRP3inflammationexpression; NLRP3SiRNA through reducing NLRP3inflammation expression to inhibit highglucose-induced cell apoptosis.