Low Oxygen Metabolism In The Lung Tissue Expression Of Glut1 And Ampk Alpha 1 And The Experimental Research Of The Role

Objective To explore the effect of one-lung ventilation on the expression of glucose transporter1(GLUT1) in lung tissue of rats.Methods30Sprague Dawley rats were subjected to anesthesia by intraperitoneal injection of10%chloral hydrate4.5mL/kg, followed by endotracheal intubation to establish OLV rat models. Rats after modeling were randomly divided into control group (n=6) and experimental group(n=24). Lung ventilation using main-stem bronchial intubation was conducted in rats in the control group, and in the experimental group, lung ventilation was performed through right main-stem bronchial intubation with no ventilation in the left lung. Rats in the experimental group were subdivided into experimental0.5h group, experimental1.0h group, experimental2.0h group and experimental4.0h group, according to the duration of non-ventilation in the left lung. Transmission electron microscopy was employed to observe the ultrastructure of alveolar epithelial cell type II (AEC II) of rats and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was adopted to detect the expression of GLUT1mRNA in lung tissue of rats.Results AEC II in control group showed nuclear chromatin with well-distribution, cytoplasmic lamellar bodies with round shape and uniform density, and clear microvilli. AEC II in each experimental group were found different degrees of changes in nuclear chromatin, cytopalsmic lamellar bodies and microvilli. Compared with the control group, expression of GLUT1mRNA was significantly decreased in lung tissue of rats in experimental0.5h group and experimental1.0h group(P <0.05), it was slightly reduced in experimental2.0h group with no statistically significance, and it was markedly increase in experimental4.0h group(P<0.05). Paired comparison among experimental groups, except the comparison between experimental1.0h group and experimental0.5h group, differences were statistically significant(P<0.05).Conclusion In the condition of hypoxia, the up-regulation of GLUT1mRNA in lung tissue possesses the protective effect on AEC Ⅱ. Objective To investigate the expression of glucose transport pro-tein (GLUT1) and AMP-activated protein kinase a1(AMPKa1) in rats’ lung tissues within one-lung ventilation.Methods30SD rats were studied in a randomized manner. After tracheotomy, rats which met predefined inclusion criteria of the weight were randomly assigned to one of five groups. Rats in the first four grou-ps (Group A、 B、 C、D) were undergoing one-lung ventilation separately within half an hour、an hour、two hours and four hours. Rats in the fifth group were executed with two-lung ventilation. One-lung ventilation mo-del was set by over deep right principal bronchus intubation with one cha-mber. There were two methods of ELISA and Western blot respectively to detect the expression of GLUT1and AMPKa1in serum and lung tissue.Results Contrasted to the controller group, there were no significant difference in the content of GLUT1and AMPKa1discharged in serum.(P>0.05)Compared to the controller group, while there was no alarming difference in the content of g and a assigned in the lung tissue.(P>0.05).Conclusions The expression of GLUT1and AMPKal are not remar- kably affected by hypoxia metabolism in predefined experimental time...