Nalp3 Renal Ischemia-reperfusion Injury In Rats In The Expression And Role Of Research

Objective To investigate the expressions of pattern recognition receptorNALP3, its effect in the kidney of rates after renal ischemia reperfusion injury (IRI)and the probable mechanism.Methods Male SD rats were divided randomly into five groups: sham group;ischemia reperfusion (I/R)1h, 2h, 4h, 8h, 16h, 24h groups; sham with pSINsi-R(emptycontrol vector) transferred group; I/R with pSINsi-R transferred group; I/R withpSINsi-F1(siRNA vector) transferred group. GroupⅠdid not receive anyischemia/reperfusion, but underwent right kidney one week before to exposure of leftrenal pedicles. GroupⅡreceive left renal I/R surgeries that reperfusion1h, 2h, 4h, 8h,16h, 24h, and underwent right kidney one week before that. GroupⅢdid not receiveany ischemia/reperfusion surgeries, but underwent right kidney and transferred anempty control vector into left renal by ultrasound-mircobubbles. GroupⅣunderwentright kidney and transferred an empty control vector into left renal byultrasound-mircobubbles and one week later receive renal I/R surgeries. GroupⅤanimals were treated the same way as groupⅣexcept that pSINsi-F1 wastransferred. Analyzed kidney histological changes semi-quantitatively. Detectedserum levels of creatinine and BUN by Beckman automatic biochemistry analyzer.Detected The protein expression of NALP3 and the phosphorylations of JNK, ERK,P38 in the kidney by Western blotting. Detected the mRNA expression of NALP3 inthe kidney by semi-quantitative reverse transcription-polymerse chain reaction(RT-PCR).Results (1) Significant pathologic changes were noticed in kidneys of renal I/Rmodel rats, and the changes were much severer at 4 h after reperfusion. (2) Serumlevels of creatinine and BUN were much higher than control group, and highest at 4hafter reperfusion. (3) The protein expression of NALP3 at 1h,2h,4h,8h,16h,24h washigher than the sham group(P＜0.05), and highest at 4h. (4) Compared with I/R4hgroup, rats in pSINsi-F1 transferred group displayed markedly a decline in totalpathological injury score, the phosphorylations of JNK and P38 produced a significantdecrease, but the phosphorylation of ERK remains highly.Conclusions 1) The protein expression of NALP3 was up-regulated in thekidney after renal I/R, and when down-regulated its expression, the pathologicalchanges was significantly ameliorated. 2) The phosphorylations of MAPKincreased in the kidney after renal I/R, and when down-regulated expression of NALP3, the phosphorylations of MAPK was markedly reduced, which suggeststhere is a protect effect in the kidney of rates after renal ischemia reperfusion injury(IRI) when down-regulated the expressions of pattern recognition receptor NALP3,which achieved by restrain the activation of JNK, P38.