Suppress A Wheatgrass Among Species Genome Diversity And Repetitive Sequence Separation And Identification Of Research

Elytrigia is a related species of wheat, which contains many resistance genes, such as powdery mildew, rust and yellow dwarf disease and so forth. It is an excellent resource for wheat breeding and genetics research.The gluten is the major storage protein in wheat, which has an important influence on wheat quality, therefore plays an important role in wheat quality improvement. In this research, we have analyzed the gluten of76Thinopyrum lines from worldwide. We found that the Thinopyrum glutenin subunit bands are not as rich as Chinese Spring and Chuanyu12. It has nine types, and the largest subunit is2*, about29.3%. The largest subunit from temperate continental climate is1*, about29.8%. In addition to some new subunit combinations, such as5*and12*,2*and10*, up to15.8%material do not contain HMW protein. Those genetic diversities are a good resource for wheat breeding.Using repeat sequence pDb12H,10Thinopyrum intermedium lines from Iran, Turkey, Kazakhstan, North Asia, Russia and other countries were analyzed by FISH.8chromosomes have strong signal in all regions besides the end and centromere, and6chromosomes have a strong signal around the centromere. We found that14chromosomes have hybridization signal in7Thinopyrum intermedium lines, indicating that the Js chromosome from different regions have a certain conservative. In contrast, the material from Iran various, in situ hybridization showed that13chromosomes and1telocentric chromosome have signals in PI401177. In PI440048,15chromosomes have signals, which contain14chromosomes and a telocentric chromosome. The variation of pDb12H hybridization signals indicates that complex chromosome rearrangement happened in the process of Thinopyrum intermedium genome evolution. The results suggest that Thinopyrum intermedium has a rich genetic diversity, which can be chosen as the donor of wheat distant hybridization and pasture breeding.E genome is the basis of Elytrigia, which is made of diploid, tetraploid hexaploid and ten times species. The hexaploid Thinopyrum intermedium is widely used in breeding. Now numerous research suggested that the hexaploid Thinopyrum intermedium chromosome is made from Js, J, St or Ee, Eb St. In this study, through the diploid fosmid library, we found3CRM-like colons, NO41, NO73and NO97. We analyzed diploid, tetraploid, hexaploid and ten times Thinopyrum using NO41and NO73as probe. In diploid and and tetraploid Thinopyrum, all chromosomes have signals in the centromeric region, whereas in hexaploid Thinopyrum,28chromosomes have signals, and14chromosomes have strong signals. This is probably because the mutual coordination and integration of St and E group in the evolution. In ten times Thinopyrum, only28chromosomes have signals, without stronger one, indicating that ten times Thinopyrum is not from hexaploid Thinopyrum intermedium but hexaploid wick Thinopyrum.In summary, Elytrigia has rich genetics diversity, and therefore is excellent resource for Wheat breeding. There is some controversy on Thinopyrum intermedium and ten times Thinopyrum genome formation. This study also give some conjecture on possible sources.