Burdock Extraction And Purification Of Polysaccharide And Its Oxidation Resistance

Dadzis,the evil is a stick, and the biennial herb with fleshy roots for the products. Dadzis polysaccharides have the capacity of tumor growth, inflation and antimicrobial resistance to fungus and function. Dadzis in long-term use for medicinal, in recent years to come to a nutritional value as food and their pharmacological research. This paper studies through different ways to extraction dadzis polysaccharides, and optimizes the extraction process; purification technology optimize; polysaccharides structure characterization of coarse polysaccharides; the oxidation of dadzis polysaccharides, no protein polysaccharides, pure polysaccharides. Results are as follows:1.Studies the hot water, ultrasound and microwave extraction the polysaccharides process. Studies solid-liquid, time,temperature and ethanol consistency of three kinds of material to extraction dadzis polysaccharides. And then, optimize the extraction process of ultrasonic, microwave, the hot water. The results showed:On the basis of solid-liquid is1:10, temperature is80℃.time is2h, the water extraction maximum rate is3.94%. On the basis of solid-liquid is1:15, time is25min, ethanol consistency is80%,the ultrasound extraction maximum rate is5.09%. On the basis of solid-liquid is1:15, time is25min, ethanol consistency is70%, the microwave extraction maximum rate is4.09%. Contrast three extraction process can be seen with ultrasound extraction dadzis polysaccharide is the highest withdrawal rates.2.Separation and purification of Dadzis polysaccharide found:Contrast enzymes and sevag, the Sevag+Enzymes has advantages, first enzymes to get proteins,then sevag to get seldom proteins. The main advantage lies in less. Enzymes the conditions is45℃,0.5%proportion,1h,pH7. Divide small molecules material use intercept measurefor3000dialysis bag for lh. The dadzis polysaccharides separate two kinds of polysaccharides by SephadexG-75. Collecting the most. By UVshows that this is a no-protein and no-carbohydrate polysaccharides, result of the Electrophores shows that is purification polysaccharides,and the luminosity is-30.80.3. Study on monosaccharide of dadzis polysaccharide found:Thin layers of disintegrating find that Dadzis polysaccharide is combined of Glucose, Fructose and half lactose.1HNMR shows dadzis polysaccharide has α and β style characterizationshowed, Polysaccharide is composed of three monosaccharide. From profile shows dadzis apolysaccharide is composed of fructose, glucose and lactose.The oxidizing of high iodine shows dadzis pure polysaccharides has1→6style,at the same time has many1→2orl→4style. The contrast ofl→and1→2or1→4style is1:1.1.4. Polysaccharides, no-protein polysaccharides and pure polysaccharides in an antioxidant experiment. Study antioxidant in the DPPH-system, antioxidant in the lard and sesame oil,the capacity of remove the oxygen the ions and·OH, restoring force.found:(1)DPPH.:When the quality proportion of polysaccharides, no-protein polysaccharides and pure polysaccharides with DPPH·is3.84,4.00,4.74,can clear50%DPPH·Means antioxidant sexual weaker one by one;(2)POV:polysaccharides, no-protein polysaccharides and pure polysaccharides weaker with antioxidant sexual, antioxidant effects were added by quatity increase, suggested adding the amount of0.05%-0.1%;(3)·OH:When the quality proportion of polysaccharides, no-protein polysaccharides and pure polysaccharides with-OH is0.28mg/mL,0.37mg/mL,0.44mg/mL,can clear50%·OH. Means antioxidant sexual weaker one by one;(4)O2·-:When the quality proportion of polysaccharides, no-protein polysaccharides and pure polysaccharides with O2·is0.34mg/mL,0.51mg/mL,0.55mg/mL,can clear50%O2·-. Means antioxidant sexual weaker one by one;(5) Restoring force:When the quality proportion of polysaccharides, no-protein polysaccharides and pure polysaccharides with restoring force is0.52mg/mL,0.57mg/mL,0.59mg/mL, the absorbency is0.5. Means antioxidant sexual weaker one by one.